Graduate School of International Agricultural Technology, Seoul National University, Pyeongchang 25354, the Republic of Korea.
Crop Biotechnology Institute, Green Bio Science and Technology, Seoul National University, Pyeongchang 25354, the Republic of Korea; School of Biological Sciences and Technology, Chonnam National University, Gwangju 61186, the Republic of Korea.
Plant Sci. 2022 Oct;323:111404. doi: 10.1016/j.plantsci.2022.111404. Epub 2022 Jul 30.
Land plants have developed a comprehensive system to cope with the drought stress, and it is operated by intricate signaling networks, including transcriptional regulation. Herein, we identified the function of OsNAC17, a member of NAC (NAM, ATAF, and CUC2) transcription factor family, in drought tolerance. OsNAC17 is localized to the nucleus, and its expression was significantly induced under drought conditions. A transactivation assay in yeast revealed that the OsNAC17 is a transcriptional activator, harboring an activation domain in the C-terminal region. Overexpressing (OsNAC17) transgenic plants showed drought-tolerant, and knock-out (OsNAC17) plants exhibited drought susceptible phenotype compared to non-transgenic plants. Further investigation revealed that OsNAC17 positively regulates several lignin biosynthetic genes and promotes lignin accumulation in leaves and roots. Together, our results show that OsNAC17 contributes to drought tolerance through lignin biosynthesis in rice.
陆生植物已经发展出了一套综合的系统来应对干旱胁迫，该系统由包括转录调控在内的复杂信号网络来操控。在此，我们鉴定了 NAC（NAM、ATAF 和 CUC2）转录因子家族成员 OsNAC17 在耐旱性中的功能。OsNAC17 定位于细胞核，其表达在干旱条件下显著诱导。酵母中的转激活测定表明，OsNAC17 是一种转录激活因子，在 C 端区域含有一个激活结构域。与非转基因植物相比，过表达（OsNAC17）转基因植物表现出耐旱性，而敲除（OsNAC17）植物表现出对干旱敏感的表型。进一步的研究表明，OsNAC17 正向调控几个木质素生物合成基因，并促进叶片和根部的木质素积累。总之，我们的结果表明，OsNAC17 通过水稻中的木质素生物合成来促进耐旱性。
