Hu Runkai, Hotta Morihiro, Maruyama Taro, Fujisawa Mizuki, Sou Keitaro, Takeoka Shinji
Department of Life Science and Medical Bioscience, Graduate School of Advanced Science and Engineering, Waseda University, Tokyo 162-8480, Japan.
Waseda Research Institute for Science and Engineering, Waseda University, Tokyo 169-8555, Japan.
ACS Omega. 2022 Jul 21;7(30):26936-26944. doi: 10.1021/acsomega.2c03597. eCollection 2022 Aug 2.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which is the etiological agent of coronavirus disease 2019 (COVID-19), has infected more than 340 million people since the outbreak of the pandemic in 2019, resulting in approximately 55 million deaths. The rapid and effective diagnosis of COVID-19 patients is vital to prevent the spread of the disease. In a previous study, we reported a novel temperature-responsive liposome-linked immunosorbent assay (TLip-LISA) using biotinylated-TLip that exhibited high detection sensitivity for the prostate-specific antigen. Herein, we used immunoglobulin-TLip (IgG-TLip), in which the antibodies were directly conjugated to the liposomal surface to simplify pretreatment procedures and reduce the detection time for SARS-CoV-2. The results indicated that TLip-LISA could detect the recombinant nucleocapsid protein and the nucleocapsid protein in inactivated virus with 20 min incubation time in total, and the limit of detection was calculated to be 2.2 and 1.0 pg/mL, respectively. Therefore, TLip-LISA has high potential to be used in clinic for rapid diagnosis and disease control.
严重急性呼吸综合征冠状病毒2（SARS-CoV-2）是2019冠状病毒病（COVID-19）的病原体，自2019年大流行爆发以来，已感染超过3.4亿人，导致约5500万人死亡。对COVID-19患者进行快速有效的诊断对于防止疾病传播至关重要。在先前的一项研究中，我们报道了一种新型的温度响应脂质体连接免疫吸附测定法（TLip-LISA），该方法使用生物素化的TLip，对前列腺特异性抗原有很高的检测灵敏度。在此，我们使用免疫球蛋白-TLip（IgG-TLip），其中抗体直接与脂质体表面偶联，以简化预处理程序并缩短SARS-CoV-2的检测时间。结果表明，TLip-LISA总共孵育20分钟就能检测重组核衣壳蛋白和灭活病毒中的核衣壳蛋白，计算得出的检测限分别为2.2和1.0 pg/mL。因此，TLip-LISA在临床上用于快速诊断和疾病控制具有很高的潜力。
