Di Domenico Marina, De Rosa Alfredo, Boccellino Mariarosaria
Department of Precision Medicine, University of Campania "Luigi Vanvitelli", 81100 Naples, Italy.
Department of Biology, College of Science and Technology, Temple University, Philadelphia, PA 19122, USA.
Diagnostics (Basel). 2021 Apr 14;11(4):698. doi: 10.3390/diagnostics11040698.
The coronavirus disease 2019 (COVID-19) global pandemic created an unprecedented public health emergency. Early recognition of an infected person and disruption of the transmission pathway are the keys to controlling this major public health threat around the world. The scientifically reliable screening method is an RT-PCR test that is performed on an ororhinopharyngeal swab in the laboratory. In the current severe SARS-CoV-2 pandemic, it is necessary to identify devices for rapid diagnosis to reduce the spread of the disease. The aim of this study was to provide a qualitative, rapid, sensitive, and specific method for a diagnosis of SARS-CoV-2 infection based on the recognition of specific antigens of the SARS-CoV-2 virus. The device was built by assembling commercially available and custom-made semi-finished products. The method was performed in environments outside the laboratory, i.e., "patient side," with an immediate chemocolorimetric response or with a digital reader using an ELISA method.
2019年冠状病毒病（COVID-19）全球大流行造成了前所未有的公共卫生紧急情况。早期识别感染者并阻断传播途径是控制全球这一重大公共卫生威胁的关键。科学可靠的筛查方法是在实验室对口咽拭子进行的逆转录聚合酶链反应（RT-PCR）检测。在当前严重的严重急性呼吸综合征冠状病毒2（SARS-CoV-2）大流行中，有必要识别用于快速诊断的设备，以减少疾病传播。本研究的目的是基于识别SARS-CoV-2病毒的特定抗原，提供一种定性、快速、灵敏且特异的SARS-CoV-2感染诊断方法。该设备通过组装市售和定制的半成品构建而成。该方法在实验室外的环境中，即“患者端”，采用即时化学比色反应或使用酶联免疫吸附测定（ELISA）方法的数字读取器进行。
