Jayahari Nidhin Kumar, Niranjan Nandini T, Kanaparthy Aruna
Department of Conservative Dentistry and Endodontics, PSM Dental College and Hospital, Kunnamkulam, Trichur, Kerala, India.
J Investig Clin Dent. 2014 May;5(2):154-60. doi: 10.1111/jicd.12023. Epub 2013 Jan 25.
To assess the effectiveness of several concentrations of two forms of passion fruit juice (PFJ) in the elimination of Enterococcus faecalis and to compare the antibacterial property of PFJ with sodium hypochlorite (NaOCl) as an intracanal irrigant.
Two types of PFJs, aqueous and alcohol extracts, were prepared and a minimum inhibitory concentration (MIC) test was performed with both the extracts against E. faecalis. Two concentrations of each extract were selected from the results given by the MIC test and subjected to a broth dilution test (BDT) for nine different time periods. After each time period, samples were inoculated in brain-heart infusion agar plates for 24 h at 37°C and results were compared statistically.
The MIC test showed that E. faecalis was sensitive to PFJ extracts at various concentrations. The results of the BDT showed a negative growth of E. faecalis by PFJ alcohol 20% at 30 min, PFJ aqueous 20% at 1 h, NaOCl 2.5% at 10 min and NaOCl 5.25% at 1 min. NaOCl showed a much better antibacterial efficacy than PFJ.
The PFJ alcoholic and aqueous extracts had an antibacterial effect against E. faecalis. As PFJ shows promising results, further research in this field could lead to much better results as compared to NaOCl.
评估两种形式的西番莲果汁(PFJ)的几种浓度对粪肠球菌的清除效果,并将PFJ与次氯酸钠(NaOCl)作为根管冲洗剂的抗菌性能进行比较。
制备了两种类型的PFJ,即水提取物和醇提取物,并对两种提取物进行了针对粪肠球菌的最低抑菌浓度(MIC)测试。从MIC测试给出的结果中选择每种提取物的两种浓度,并对其进行九个不同时间段的肉汤稀释试验(BDT)。在每个时间段后,将样品接种于脑心浸液琼脂平板中,在37℃下培养24小时,并对结果进行统计学比较。
MIC测试表明,粪肠球菌对不同浓度的PFJ提取物敏感。BDT结果显示,20%的PFJ醇提取物在30分钟时使粪肠球菌生长呈阴性,20%的PFJ水提取物在1小时时使粪肠球菌生长呈阴性,2.5%的NaOCl在10分钟时使粪肠球菌生长呈阴性,5.25%的NaOCl在1分钟时使粪肠球菌生长呈阴性。NaOCl显示出比PFJ更好的抗菌效果。
PFJ醇提取物和水提取物对粪肠球菌有抗菌作用。由于PFJ显示出有前景的结果,与NaOCl相比,该领域的进一步研究可能会带来更好的结果。