Lazcano-Silveira Rayko, Jia Xiaoxiao, Liu Kaixuan, Liu Honggang, Li Xinrong, Hui Mizhou
College of Life Sciences, Northeast Agricultural University, Harbin, Heilongjiang, People's Republic of China.
College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, Shandong, People's Republic of China.
J Inflamm Res. 2022 Jul 25;15:4185-4198. doi: 10.2147/JIR.S366886. eCollection 2022.
Carbon 60 (C60) and its derivatives have various biological applications. In our laboratory, we have demonstrated that C60 dissolved in grape seed oil (C60-Oil) has antioxidant and anti-inflammatory properties; however, the effectiveness of this formulation to treat diseases of the intestinal tract and specifically ulcerative colitis has not been studied. In this study, we intend to explore the effects of C60-Oil against experimental ulcerative colitis induced by Dextran Sulfate Sodium (DSS) in rats and a human colorectal cell line, HT-29.
The rats were randomly distributed into three groups: a negative control group with no induced damage and two other groups were treated with DSS to induce UC for seven days: one as untreated control and the other group treated with C60-Oil 3 mg/kg/day. We quantified the clinical manifestations of the disease, body weight, colon weight, microscopic damage score, and colonic content of IL-6, TNF-alpha, IL-1B, and IL-10. As part of the cell studies, HT-29 cells were pretreated with C60-Oil at different concentrations (0.1, 1, 5, 10, 50, 30 μg/mL) and then stimulated with DSS (10 μg/mL). We measured the levels of IL-8 and NO secreted in the medium and the intracellular levels of ROS.
Oral treatment with C60-Oil significantly prevented the change in body weight, reduced most of the clinical signs of the disease, colon weight, microscopic damage score, and considerably improved the profile of cytokines analyzed. The pretreatment of HT-29 cells also protected the cells from the action of DSS as it reduced the levels of IL-8, NO, and ROS.
According to our results, we can suggest C60-Oil, as a formulation with pharmacological potential for treating ulcerative colitis.
碳60（C60）及其衍生物具有多种生物学应用。在我们实验室，我们已证明溶解于葡萄籽油中的C60（C60 - 油）具有抗氧化和抗炎特性；然而，该制剂治疗肠道疾病尤其是溃疡性结肠炎的有效性尚未得到研究。在本研究中，我们旨在探究C60 - 油对葡聚糖硫酸钠（DSS）诱导的大鼠实验性溃疡性结肠炎以及人结肠癌细胞系HT - 29的影响。
将大鼠随机分为三组：一组为无诱导损伤的阴性对照组，另外两组用DSS诱导UC七天：一组为未治疗对照组，另一组用3毫克/千克/天的C60 - 油治疗。我们对疾病的临床表现、体重、结肠重量、微观损伤评分以及结肠中白细胞介素 - 6（IL - 6）、肿瘤坏死因子 - α（TNF - α）、白细胞介素 - 1β（IL - 1B）和白细胞介素 - 10（IL - 10）的含量进行了量化。作为细胞研究的一部分用不同浓度（0.1、1、5、10、50、30微克/毫升）的C60 - 油预处理HT - 29细胞，然后用DSS（10微克/毫升）刺激。我们测量了培养基中分泌的白细胞介素 - 8（IL - 8）和一氧化氮（NO）水平以及细胞内活性氧（ROS）水平。
口服C60 - 油显著预防了体重变化，减轻了疾病的大多数临床症状、结肠重量、微观损伤评分，并显著改善了所分析细胞因子的情况。HT - 29细胞的预处理也保护细胞免受DSS的作用，因为它降低了IL - 8、NO和ROS的水平。
根据我们的结果，我们可以认为C60 - 油作为一种具有治疗溃疡性结肠炎药理学潜力的制剂。
