Central Laboratory, The First Affiliated Hospital of Hebei North University, Zhangjiakou, China.
Department of Immunology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, China.
J Med Virol. 2022 Dec;94(12):5627-5639. doi: 10.1002/jmv.28040. Epub 2022 Aug 10.
Mammalian chromosomes undergo varying degrees of compression to form three-dimensional genome structures. These three-dimensional structures undergo dynamic and precise chromatin interactions to achieve precise spatial and temporal regulation of gene expression. Most eukaryotic DNA viruses can invade their genomes into the nucleus. However, it is still poorly understood how the viral genome is precisely positioned after entering the host cell nucleus to find the most suitable location and whether it can specifically interact with the host genome to hijack the host transcriptional factories or even integrate into the host genome to complete its transcription and replication rapidly. Chromosome conformation capture technology can reveal long-range chromatin interactions between different chromosomal sites in the nucleus, potentially providing a reference for viral DNA-host chromatin interactions. This review summarized the research progress on the three-dimensional interaction between virus and host genome and the impact of virus integration into the host genome on gene transcription regulation, aiming to provide new insights into chromatin interaction and viral gene transcription regulation, laying the foundation for the treatment of infectious diseases.
哺乳动物染色体经历不同程度的压缩，以形成三维基因组结构。这些三维结构经历动态和精确的染色质相互作用，以实现基因表达的精确时空调节。大多数真核 DNA 病毒可以将其基因组侵入细胞核。然而，病毒基因组进入宿主细胞后如何精确定位以找到最合适的位置，以及它是否可以与宿主基因组特异性相互作用以劫持宿主转录工厂，甚至整合到宿主基因组中以快速完成转录和复制，目前仍知之甚少。染色质构象捕获技术可以揭示核内不同染色体位点之间的长距离染色质相互作用，为病毒 DNA-宿主染色质相互作用提供参考。本综述总结了病毒与宿主基因组之间的三维相互作用以及病毒整合到宿主基因组对基因转录调控的影响的研究进展，旨在为染色质相互作用和病毒基因转录调控提供新的见解，为传染病的治疗奠定基础。
