Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, People's Republic of China.
Avian Pathol. 2022 Dec;51(6):574-589. doi: 10.1080/03079457.2022.2109453. Epub 2022 Oct 24.
Baicalin, a flavonoid compound extracted from the dry root of Georgi, has been shown to have anti-inflammation, anti-viral, anti-bacterial, and immunomodulatory activity. However, the effect of baicalin against avian infectious bronchitis virus (IBV) remains unknown. The purpose of this study was to investigate the anti-IBV activity and underlying mechanism of baicalin . The results showed that baicalin has a direct virucidal effect but no prophylactic effect on IBV infection. The mRNA and protein of IBV N were decreased significantly when IBV-infected cells were treated with baicalin during the multiple stages of the virus replication cycle, including viral adsorption, invasion, internalization, and release. Stress granule (SG) formation resulted from the increase of G3BP1 and the phosphorylation of the PKR/eIF2α due to the treatment of IBV-infected cells with baicalin. The inhibitory activity of baicalin on IBV replication was increased when G3BP1 expression was inhibited, and the down-regulation of G3BP1 expression occurred when the expression of PKR and eIF2α was inhibited. These findings revealed that baicalin activates phosphorylation of the PKR/eIF2α pathway and induces SG formation by targeting G3BP1, initiating the antiviral response to suppress IBV replication in Vero cells. The results suggest that baicalin is a promising candidate drug to treat or prevent IBV infection. Baicalin inhibits IBV replication by reducing IBV N protein and mRNA.Baicalin disturbs multiple stages of the IBV life cycle.Baicalin activates PKR/eIF2α pathway and induces stress granule formation to exert anti-IBV response.
黄芩苷是从黄芩的干燥根中提取的一种黄酮类化合物，具有抗炎、抗病毒、抗菌和免疫调节活性。然而，黄芩苷对禽传染性支气管炎病毒（IBV）的作用尚不清楚。本研究旨在研究黄芩苷对 IBV 的抗病毒活性及其作用机制。结果表明，黄芩苷对 IBV 具有直接的杀病毒作用，但对 IBV 感染没有预防作用。当 IBV 感染细胞在病毒复制周期的多个阶段（包括病毒吸附、入侵、内化和释放）用黄芩苷处理时，IBV N 的 mRNA 和蛋白显著降低。由于黄芩苷处理 IBV 感染细胞，导致 G3BP1 增加和 PKR/eIF2α 的磷酸化，从而形成应激颗粒（SG）。当抑制 G3BP1 的表达时，黄芩苷抑制 IBV 复制的活性增加，而当抑制 PKR 和 eIF2α 的表达时，G3BP1 的表达下调。这些发现表明，黄芩苷通过靶向 G3BP1 激活 PKR/eIF2α 通路并诱导 SG 形成，从而引发抗病毒反应，抑制 Vero 细胞中 IBV 的复制。研究结果表明，黄芩苷是治疗或预防 IBV 感染的一种有前途的候选药物。黄芩苷通过降低 IBV N 蛋白和 mRNA 抑制 IBV 复制。黄芩苷扰乱 IBV 生命周期的多个阶段。黄芩苷激活 PKR/eIF2α 通路并诱导应激颗粒形成，从而发挥抗 IBV 反应。
