Nolan J P, DiBenedetto G, Tarsa N J
Clin Chem. 1981 May;27(5):738-41.
We have evaluated a fully automated thyroxine assay involving the use of a homogeneous enzyme assay and a Technicon AutoAnalyzer II continuous flow system. Comparison by regression analysis with a thyroxine radioimmunoassay kit method gave a slope of 0.82 and a y-intercept of 7.81 micrograms/L (SE 0.86 microgram/L, r = 0.95). Within-run precision yielded CVs of 1.0-6.1%, between-day CVs were 2.0-14.4%; within-day precision showed a mean variance of 7.81 micrograms/L. Mean analytical recovery was 96.1%. Bilirubin, hemoglobin, and lipemia interfered with quantitation of results when their concentrations exceeded 50 mg/L, 300 mg/L, and 5 g/L, respectively. The reference interval for euthyroid status was calculated to be 50-110 micrograms/L. Sensitivity was 5.0 micrograms/L with a mean carryover of 1.65%. Current reagent and labor costs for enzyme immunoassay ($0.40) were less than for the manual radioimmunoassay procedure ($0.40) were less than for the manual radioimmunoassay procedure ($1.70). The assay is economical, simple to perform, and amenable to high-throughput thyroid screening in the routine laboratory.
我们评估了一种全自动甲状腺素检测方法，该方法采用均相酶检测法和Technicon AutoAnalyzer II连续流动系统。通过回归分析与甲状腺素放射免疫分析试剂盒方法进行比较，得到斜率为0.82，y轴截距为7.81微克/升（标准误0.86微克/升，r = 0.95）。批内精密度的变异系数为1.0 - 6.1%，日间变异系数为2.0 - 14.4%；日内精密度显示平均方差为7.81微克/升。平均分析回收率为96.1%。当胆红素、血红蛋白和脂血的浓度分别超过50毫克/升、300毫克/升和5克/升时，会干扰结果的定量。甲状腺功能正常状态的参考区间经计算为50 - 110微克/升。灵敏度为5.0微克/升，平均残留率为1.65%。酶免疫分析当前的试剂和劳动力成本（0.40美元）低于手工放射免疫分析程序（1.70美元）。该检测方法经济、操作简单，适用于常规实验室的高通量甲状腺筛查。
