Department of Thoracic Surgery, Erzincan Binali Yildirim University Faculty of Medicine, Erzincan-Türkiye.
Department of Pharmacology, Erzincan Binali Yildirim University Faculty of Medicine, Erzincan-Türkiye.
Ulus Travma Acil Cerrahi Derg. 2022 Aug;28(8):1073-1081. doi: 10.14744/tjtes.2021.97760.
Acute cardiac contusion induced by trauma is known with its high mortality and morbidity. The role of oxidative stress and inflammation in its pathophysiology has led to the investigation of antioxidant and anti-inflammatory substances in non-sur-gical treatment. In this study, the effects of rutin which has these two features on acute cardiac contusion were investigated.
Thirty male albino Wistar rats were divided into three equal groups as healthy (HG), contusion (CG), and rutin + con-tusion (rutin + CG). A heart contusion was created dropping 200 g weight from 1-m height onto anterior thorax of CG (n=10) and Rutin + CG (n=10) group animals by anesthetizing with intraperitoneal administration of 60 mg/kg ketamine and xylazine inhalation at appropriate intervals. Thirty minutes after contusion was applied, rutin at the dose of 50 mg/kg was administered orally to the stomach by gavage to the rutin + CG group animals. The rutin was used once a day for 2 days. Rats were killed at the end of 48 h. Heart tissues were removed and examined biochemically and histopathologically. Troponin I (TP I) and creatine kinase-MB (CK-MB) were measured in blood samples taken from the tail veins just before the rats were killed.
TP I, CK-MB, malondialdehyde, total oxidant status, and nuclear factor-kappa B levels increased in the CG when compared to the HG, and Rutin application prevented this increase, total glutathione (eGSH) and total antioxidant status levels decreased, and rutin application prevented this decrease. Histopathological findings also supported these findings.
Rutin had a protective effect on heart tissue.
创伤引起的急性心脏挫伤具有较高的死亡率和发病率。氧化应激和炎症在其病理生理学中的作用促使人们研究非手术治疗中的抗氧化和抗炎物质。在这项研究中，研究了具有这两种特性的芦丁对急性心脏挫伤的影响。
将 30 只雄性白化 Wistar 大鼠随机分为三组：健康组（HG）、挫伤组（CG）和芦丁+挫伤组（芦丁+ CG）。通过腹腔注射 60mg/kg 氯胺酮和适当间隔吸入二甲苯麻醉，将 200g 重量从 1m 高度滴落在 CG（n=10）和芦丁+ CG（n=10）组动物的前胸部，造成心脏挫伤。挫伤后 30 分钟，通过灌胃向芦丁+ CG 组动物的胃中给予芦丁 50mg/kg 一次。芦丁每天使用一次，共使用两天。在 48 小时结束时处死大鼠。取出心脏组织进行生物化学和组织病理学检查。在处死大鼠之前，从尾静脉采集血液样本，测量肌钙蛋白 I（TP I）和肌酸激酶同工酶-MB（CK-MB）。
与 HG 相比，CG 中的 TP I、CK-MB、丙二醛、总氧化剂状态和核因子-κB 水平升高，芦丁应用可防止这种升高，总谷胱甘肽（eGSH）和总抗氧化状态水平降低，芦丁应用可防止这种降低。组织病理学发现也支持这些发现。
芦丁对心脏组织具有保护作用。
