Elma Bekir, Mammadov Renad, Bilgin Yasin, Yazıcı Gülce Naz, Süleyman Halis, Çoban Taha Abdulkadir
Department of Thoracic Surgery, Medicine Faculty of Erzincan Binali Yıldırım University, Erzincan, Türkiye.
Department of Pharmacology, Medicine Faculty of Erzincan Binali Yıldırım University, Erzincan, Türkiye.
Turk Gogus Kalp Damar Cerrahisi Derg. 2022 Jul 29;30(3):431-439. doi: 10.5606/tgkdc.dergisi.2022.21992. eCollection 2022 Jul.
This study aims to investigate the possible protective effects of rutin, also called vitamin P1, against pulmonary contusion induced by blunt chest trauma in a rat model.
Thirty male albino Wistar rats were separated into three equal groups as healthy group, trauma group, and trauma+rutin group. After anesthesia provided by intraperitoneal administration of 60 mg/kg ketamine and xylazine by inhalation at appropriate intervals, 200 g weight was dropped from 1 m height to the anterior chest wall of the animals in the trauma group (n=10) and trauma+rutin group (n=10) and pulmonary contusion was created. Thirty min after the trauma, 50 mg/kg of rutin was administered into the stomach of trauma+rutin group animals orally with gavage. The rats received rutin once daily for two days and were sacrificed 48 h later. Their lung tissues were removed and examined biochemically and histopathologically.
Nuclear factor-kappa B, cyclooxygenase-2, and malondialdehyde levels increased in the trauma group compared to the healthy group, and rutin administration prevented this increase. Total glutathione levels decreased in the trauma group, and rutin administration also prevented this decrease. The histopathological findings were compatible with the biochemical findings.
Our study results suggest that rutin has a protective effect on contused lung tissue in rats.
本研究旨在探讨芦丁（又称维生素P1）对大鼠钝性胸部创伤所致肺挫伤可能的保护作用。
将30只雄性白化Wistar大鼠分为三组，每组10只，分别为健康组、创伤组和创伤+芦丁组。经腹腔注射60mg/kg氯胺酮麻醉，并适时吸入甲苯噻嗪后，将200g重物从1m高度落至创伤组（n=10）和创伤+芦丁组（n=10）动物的前胸壁，造成肺挫伤。创伤后30分钟，经口灌胃给予创伤+芦丁组动物50mg/kg芦丁。大鼠连续两天每日接受一次芦丁，48小时后处死。取出其肺组织进行生化和组织病理学检查。
与健康组相比，创伤组核因子-κB、环氧化酶-2和丙二醛水平升高，而给予芦丁可防止这种升高。创伤组总谷胱甘肽水平降低，给予芦丁也可防止这种降低。组织病理学结果与生化结果相符。
我们的研究结果表明，芦丁对大鼠肺挫伤组织具有保护作用。
