Topal İsmail, Özbek Bilgin Aslı, Keskin Çimen Ferda, Kurt Nezahat, Süleyman Zeynep, Bilgin Yasin, Özçiçek Adalet, Altuner Durdu
Department of Pharmacology, Faculty of Medicine, Erzincan University; Erzincan-Turkey.
Anatol J Cardiol. 2018 Sep;20(3):136-142. doi: 10.14744/AnatolJCardiol.2018.32708.
Cisplatin is an anticancer drug used for treating childhood solid tumors. Symptoms related to cisplatin-induced cardiovascular adverse effects may be mild or severe. Rutin (vitamin P1) has many properties, including as antioxidant, anticancer, antidiabetic, antimicrobial, antiulcer, and tissue renewal properties. Therefore, we aimed to biochemically, histopathologically, and immunohistochemically demonstrate the effect of rutin on cisplatin-induced cardiotoxicity in rats.
The rats included in our study were divided into four groups: Healthy group (HE), 5-mg/kg cisplatin group (CP), 50 mg/kg rutin+5-mg/kg cisplatin (CR-50), 100-mg/kg rutin+5-mg/kg cisplatin (CR-100) group.
CP group administered cisplatin had significantly increased blood, serum, and cardiac tissue malondialdehyde (MDA), interleukin 1 beta (IL-1ß), tumor necrosis factor alpha (TNF-α), troponin I, creatine kinase (CK), and CK-MB levels compared to the HE group, whereas there was a significant decrease in the total glutathione (tGSH) levels. Rutin was observed to prevent the increase in MDA, IL-1ß, TNF-α, troponin I, CK, and CK-MB levels as well as prevent the decrease in tGSH levels more significantly when administered at a 100-mg/kg dose than at a 50-mg/kg dose. Histopathologically, cardiac necrosis, dilated/congested blood vessels, hemorrhage, polymorphonuclear leukocyte, edema, and cells with pyknotic nuclei were observed in the CP group. Rutin was shown to prevent cisplatin-induced cardiac damage more effectively when used at a100-mg/kg dose than at a 50-mg/kg dose.
These results suggest that rutin is useful for preventing cisplatin-related cardiovascular damage.
顺铂是一种用于治疗儿童实体瘤的抗癌药物。与顺铂诱导的心血管不良反应相关的症状可能轻微或严重。芦丁（维生素P1）具有多种特性，包括抗氧化、抗癌、抗糖尿病、抗菌、抗溃疡和组织更新特性。因此，我们旨在通过生物化学、组织病理学和免疫组织化学方法证明芦丁对顺铂诱导的大鼠心脏毒性的影响。
纳入本研究的大鼠分为四组：健康组（HE）、5mg/kg顺铂组（CP）、50mg/kg芦丁+5mg/kg顺铂组（CR-50）、100mg/kg芦丁+5mg/kg顺铂组（CR-100）。
与HE组相比，给予顺铂的CP组血液、血清和心脏组织中的丙二醛（MDA）、白细胞介素1β（IL-1β）、肿瘤坏死因子α（TNF-α）、肌钙蛋白I、肌酸激酶（CK）和CK-MB水平显著升高，而总谷胱甘肽（tGSH）水平显著降低。观察到，与50mg/kg剂量相比，芦丁以100mg/kg剂量给药时，更能显著预防MDA、IL-1β、TNF-α、肌钙蛋白I、CK和CK-MB水平的升高以及tGSH水平的降低。组织病理学上，CP组观察到心脏坏死、血管扩张/充血、出血、多形核白细胞、水肿和核固缩细胞。与50mg/kg剂量相比，芦丁以100mg/kg剂量使用时，更能有效预防顺铂诱导的心脏损伤。
这些结果表明，芦丁有助于预防顺铂相关的心血管损伤。
