School of Biology and Food Engineering, Changshu Institute of Technology, Changshu, 215500, Jiangsu, China.
Department of Pharmacy, The First Affiliated Hospital of Soochow University, Suzhou, 215006, Jiangsu, China.
Chem Biodivers. 2022 Aug;19(8):e202200219. doi: 10.1002/cbdv.202200219. Epub 2022 Aug 3.
Sagittaria trifolia tuber is an aquatic vegetable. In this work, microwave-assisted enzymatic extraction (MEE) was used to extract S. trifolia tuber polysaccharides (STTPs). Optimum conditions were complex enzyme of 2 %, liquid-to-solid ratio of 43 : 1 mL g , microwave power of 506 W, and time of 8 min, under which STTPs yield was 36.22±0.69 %, higher than those of other methods. STTPs were sulfated polysaccharides with sulfur valence of S . STTPs comprised mannose, glucose, galactose, and arabinose at a mole ratio of 3.69 : 19.33 : 6.21 : 1.00, molecular weights of 3606 kDa and 149.6 kDa, particle size of 220 nm, and zeta potential of -5.02 mV. The surface of STTPs was full of bumps and holes, and abundant in O1s and non-functionalized C1s. STTPs would scavenge reactive oxygen species with advantage. It would provide an efficient MEE method to obtain antioxidant STTPs, also a clue for extracting polysaccharides from starch-rich crops.
三叶茨菇块茎是一种水生蔬菜。在这项工作中，采用微波辅助酶解提取法（MEE）提取三叶茨菇块茎多糖（STTPs）。最佳条件为复合酶用量 2%、液料比 43∶1 mL/g、微波功率 506 W、时间 8 min，在此条件下 STTPs 得率为 36.22±0.69%，高于其他方法。STTPs 是含硫的多糖，硫的价态为 S。STTPs 由摩尔比为 3.69∶19.33∶6.21∶1.00 的甘露糖、葡萄糖、半乳糖和阿拉伯糖组成，分子量分别为 3606 kDa 和 149.6 kDa，粒径为 220 nm，Zeta 电位为-5.02 mV。STTPs 表面布满凹凸，富含 O1s 和非功能化的 C1s。STTPs 具有清除活性氧的优势。它为获得抗氧化 STTPs 提供了一种有效的 MEE 方法，也为从富含淀粉的作物中提取多糖提供了线索。
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