School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
Carbohydr Polym. 2020 Feb 1;229:115355. doi: 10.1016/j.carbpol.2019.115355. Epub 2019 Sep 23.
To ascertain the preliminary structural characteristics and biological activity of different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs) by ultrasonic assisted extraction (UAE), four new polysaccharides (SPC-60, SPC-70, SPC-80 and SPC-90) were successively fractionated by ethanol. The results implied that except for neutral sugar, four contained proteins and uronic acids in their structure, which were further confirmed by the ultraviolet and infrared spectra. Molecular weight and monosaccharide composition analysis exhibited that SPC-60 (52.0 kDa), SPC-70 (294.9 kDa), SPC-80 (230.6 kDa) and SPC-90 (229.4 kDa) were a neutral polysaccharide composed of rhamnose, arabinose, xylose, mannose, glucose and galactose with dramatically different mole ratios. In addition, SPC-70 exhibited stronger antioxidant activity in vitro than the other three components. SPCs could significantly promote macrophage proliferation, NO release and phagocytosis. Thus, these results provided a reference for applications of S. sagittifolia L. polysaccharides which would benefit the development of industry and agriculture.
为了通过超声辅助提取（UAE）确定来自箭叶泽泻（Sagittaria sagittifolia L.）（SPCs）的多糖的不同级分的初步结构特征和生物活性，通过乙醇依次分离出四种新的多糖（SPC-60、SPC-70、SPC-80 和 SPC-90）。结果表明，除中性糖外，这四种结构中均含有蛋白质和糖醛酸，这进一步通过紫外和红外光谱得到证实。分子量和单糖组成分析表明，SPC-60（52.0 kDa）、SPC-70（294.9 kDa）、SPC-80（230.6 kDa）和 SPC-90（229.4 kDa）是由鼠李糖、阿拉伯糖、木糖、甘露糖、葡萄糖和半乳糖组成的中性多糖，其摩尔比差异很大。此外，SPC-70 在体外表现出比其他三种成分更强的抗氧化活性。SPCs 可显著促进巨噬细胞增殖、NO 释放和吞噬作用。因此，这些结果为箭叶泽泻多糖的应用提供了参考，这将有利于工农业的发展。
