School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China; Institute of Food Physical Processing, Jiangsu University, Zhenjiang 212013, China.
Int J Biol Macromol. 2019 Aug 1;134:172-179. doi: 10.1016/j.ijbiomac.2019.05.047. Epub 2019 May 7.
In the present study, orthogonal experiment (L (3)) was used to optimize the extraction process of Sagittaria sagittifolia L. polysaccharides (SSP) with subcritical water, and the antioxidant activities of polysaccharides were also investigated. The results showed that the optimum extraction conditions were obtained when pH, extraction temperature, extraction time, and liquid to solid ratio were 7, 170 °C, 16 min and 30:1 (mL/g), respectively. In addition, DPPH/ABTS radical scavenging rate and Fe reducing power of polysaccharides exhibited a certain dose-effect relationship. Furthermore, both yield and antioxidant activities of polysaccharides with subcritical water extraction (SWE) were higher than traditional hot water extraction (HWE). The above results showed that SWE was an effective technique to extract and separate polysaccharides from Sagittaria sagittifolia L., which may be potentially applied in the related polysaccharides extraction.
在本研究中，采用正交实验（L (3)）对亚临界水提取箭叶慈姑多糖（SSP）的工艺进行了优化，并对多糖的抗氧化活性进行了研究。结果表明，当 pH 值、提取温度、提取时间和液固比分别为 7、170°C、16 分钟和 30:1（mL/g）时，可获得最佳提取条件。此外，多糖对 DPPH/ABTS 自由基清除率和 Fe 还原能力表现出一定的剂量效应关系。此外，亚临界水提取（SWE）多糖的产率和抗氧化活性均高于传统热水提取（HWE）。上述结果表明，SWE 是一种从箭叶慈姑中提取和分离多糖的有效技术，可能在相关多糖提取中具有潜在的应用价值。
