Johnson Shane B, Cureoglu Sebahattin, O'Malley Jennifer T, Santi Peter A
*Department of Otolaryngology, University of Minnesota, Minneapolis, Minnesota; and †Massachusetts Eye and Ear Infirmary, Boston, Massachusetts, U.S.A.
Otol Neurotol. 2014 Aug;35(7):1145-9. doi: 10.1097/MAO.0000000000000416.
Thin-sheet laser imaging microscopy (TSLIM) optical sectioning can be used to assess temporal bone soft tissue morphology before celloidin sectioning.
Traditional human temporal bone (TB) celloidin embedding and sectioning is a lengthy and involved process. Although bone morphology can be assessed with microCT before traditional histology, soft tissue structures are difficult to resolve until after celloidin sectioning. A potential solution is TSLIM, a high-resolution, nondestructive optical sectioning technique first developed to image bone and soft tissue in animal cochleae.
Two temporal bones from 1 individual were used to evaluate TSLIM's capacity to image human temporal bones (bone and soft tissue) before traditional histology. The right TB was trimmed to the cochlea, prepared for and imaged with TSLIM, then processed for celloidin sectioning. The left TB, serving as a control, was directly prepared for traditional celloidin sectioning.
TSLIM imaging of the right TB showed adequate resolution of all major tissue structures but barely resolved cells. Celloidin sections produced from the TSLIM-imaged right TB were equivalent in cytologic detail to those from the traditionally prepared left TB. TSLIM 3-dimensional (3D) reconstructions were superior to those obtained from celloidin sections because TSLIM produced many more sections that were without mechanical sectioning artifacts or alignment issues.
TSLIM processing disturbs neither gross nor detailed morphology and integrates well with celloidin histology, making it an ideal method to image soft tissue before celloidin sectioning.
薄片激光成像显微镜（TSLIM）光学切片可用于在火棉胶切片之前评估颞骨软组织形态。
传统的人类颞骨（TB）火棉胶包埋和切片是一个漫长且复杂的过程。尽管在传统组织学检查之前可以用微型计算机断层扫描（microCT）评估骨形态，但直到火棉胶切片之后才能清晰分辨软组织结构。一种可能的解决方案是TSLIM，这是一种高分辨率、非破坏性的光学切片技术，最初是为对动物耳蜗中的骨和软组织进行成像而开发的。
使用来自1名个体的两块颞骨来评估TSLIM在传统组织学检查之前对人类颞骨（骨和软组织）进行成像的能力。将右侧颞骨修剪至耳蜗，为TSLIM成像做准备并进行成像，然后进行火棉胶切片处理。作为对照的左侧颞骨直接进行传统的火棉胶切片制备。
右侧颞骨的TSLIM成像显示所有主要组织结构都有足够的分辨率，但几乎无法分辨细胞。从经TSLIM成像的右侧颞骨制作的火棉胶切片在细胞学细节上与传统制备的左侧颞骨切片相当。TSLIM三维（3D）重建优于从火棉胶切片获得的重建，因为TSLIM产生了更多没有机械切片伪影或对齐问题的切片。
TSLIM处理既不干扰大体形态也不干扰详细形态，并且与火棉胶组织学很好地整合，使其成为在火棉胶切片之前对软组织进行成像的理想方法。
