Nair M S, Huang Y, Weathers P J
Aaron Diamond AIDS Research Center, Columbia University Irving Medical Center, New York, NY, USA.
Department of Biology and Biotechnology, Worcester Polytechnic Institute, Worcester, MA 01609, USA.
bioRxiv. 2022 Jul 25:2022.07.22.501141. doi: 10.1101/2022.07.22.501141.
The SARS-CoV-2 (COVID-19) global pandemic continuous to infect and kill millions while rapidly evolving new variants that are more transmissible and evading vaccine-elicited antibodies. L. extracts have shown potency against all previously tested variants. Here we further queried extract efficacy against omicron and its recent subvariants. Using Vero E6 cells, we measured the efficacy (IC ) of stored (frozen) dried-leaf hot-water L. extracts of four cultivars (A3, BUR, MED, and SAM) against SARS-CoV-2 variants: original WA1 (WT), BA.1.1.529+R346K (omicron), BA.2, BA.2.12.1, and BA.4. IC values normalized to the extract artemisinin (ART) content ranged from 0.5-16.5 µM ART. When normalized to dry mass of the extracted leaves, values ranged from 20-106 µg. Although IC values for these new variants are slightly higher than those reported for previously tested variants, they were within limits of assay variation. There was no measurable loss of cell viability at leaf dry weights ≤50 µg of any cultivar extract. Results continue to indicate that oral consumption of hot-water extracts (tea infusions) could potentially provide a cost-effective approach to help stave off this pandemic virus and its rapidly evolving variants.
严重急性呼吸综合征冠状病毒2（SARS-CoV-2，即新冠病毒）全球大流行仍在持续感染并导致数百万人死亡，同时还在迅速演变出传播性更强且能逃避疫苗诱导抗体的新变种。罗勒提取物已显示出对所有先前测试变种的效力。在此，我们进一步探究了提取物对奥密克戎及其近期亚变种的疗效。我们使用非洲绿猴肾细胞（Vero E6），测量了四个品种（A3、BUR、MED和SAM）的储存（冷冻）干燥叶热水罗勒提取物对SARS-CoV-2变种的疗效（半数抑制浓度[IC₅₀]）：原始的WA1（野生型）、BA.1.1.529+R346K（奥密克戎）、BA.2、BA.2.12.1和BA.4。将IC₅₀值归一化至提取物青蒿素（ART）含量后，范围为0.5 - 16.5微摩尔ART。当归一化至提取叶片的干重时，值范围为20 - 106微克。尽管这些新变种的IC₅₀值略高于先前测试变种所报告的值，但它们在测定变异范围内。在任何品种提取物的叶干重≤50微克时，均未检测到细胞活力的损失。结果继续表明，口服热水提取物（茶浸液）可能提供一种具有成本效益的方法，以帮助抵御这种大流行病毒及其迅速演变的变种。
