Institute for Translational Medicine, Qingdao University, Qingdao 266000, China.
School of Basic Medicine, Qingdao University, Qingdao 266000, China.
Biomed Res Int. 2022 Jul 25;2022:7281120. doi: 10.1155/2022/7281120. eCollection 2022.
MRG-binding protein (MRGBP) is a transcription factor widely involved in physiological and pathological processes. Many studies have discussed the relationship between the expression level of MRGBP and the prognosis of various malignant tumours. However, the role and clinicopathological significance of MRGBP in head and neck squamous cell carcinoma (HNSC) are unclear. In this study, the Wilcoxon signed-rank test and logistic regression were used to analyze the relationship between clinical characteristics and MRGBP expression in HNSC. The Kaplan-Meier plotter analysis and Cox regression analysis were established to evaluate the effect of MRGBP on prognosis, and the receiver operating characteristic (ROC) curve and nomogram was constructed. Gene set enrichment analysis (GSEA) and single-sample gene set enrichment analysis (ssGSEA) were used to analyze the correlation between MRGBP and immune infiltration. The results showed that the expression of MRGBP in HNSC tissues was significantly higher than that in normal tissues. The KM plotter analysis showed that the OS of HNSC patients was shorter. The multivariate Cox analysis further confirmed that increased expression of MRGBP was an independent risk factor for OS in HNSC patients. In addition, ROC analysis confirmed its diagnostic value and constructed prognostic nomograms, including age, T, M, N classification, pathological stage, and MRGBP. GSEA showed that MRGBP was associated with high expression of GPCR ligand binding, interleukin receptor binding, and neutrophil degranulation, and ssGSEA showed that MRGBP was associated with T cells and mast cells. In conclusion, MRGBP can serve as an independent prognostic biomarker related to immune invasion of head and neck squamous cell carcinoma.
MRG 结合蛋白（MRGBP）是一种广泛参与生理和病理过程的转录因子。许多研究讨论了 MRGBP 的表达水平与各种恶性肿瘤预后之间的关系。然而，MRGBP 在头颈部鳞状细胞癌（HNSC）中的作用和临床病理意义尚不清楚。在这项研究中，采用 Wilcoxon 符号秩检验和逻辑回归分析了 HNSC 中临床特征与 MRGBP 表达之间的关系。建立 Kaplan-Meier 绘线器分析和 Cox 回归分析来评估 MRGBP 对预后的影响，并构建了接收器操作特征（ROC）曲线和列线图。基因集富集分析（GSEA）和单样本基因集富集分析（ssGSEA）用于分析 MRGBP 与免疫浸润的相关性。结果表明，HNSC 组织中 MRGBP 的表达明显高于正常组织。KM 绘线器分析表明 HNSC 患者的 OS 更短。多变量 Cox 分析进一步证实，MRGBP 表达增加是 HNSC 患者 OS 的独立危险因素。此外，ROC 分析证实了其诊断价值，并构建了包括年龄、T、M、N 分类、病理分期和 MRGBP 在内的预后列线图。GSEA 显示 MRGBP 与 GPCR 配体结合、白细胞介素受体结合和嗜中性粒细胞脱颗粒的高表达相关，ssGSEA 显示 MRGBP 与 T 细胞和肥大细胞相关。总之，MRGBP 可以作为与头颈部鳞状细胞癌免疫浸润相关的独立预后生物标志物。
