Ferreira Andressa Coelho, Bomfim Maria Rosa Quaresma, da Costa Sobrinho Carlos Henrique de Barros, Boaz Daniela Talissa Lobo, Da Silva Lira Railane, Fontes Valéria Costa, Arruda Mariana Oliveira, Zago Patrícia Maria Wiziack, Filho Carlos Alberto Alves Dias, Dias Carlos José Moraes, da Rocha Borges Marilene Oliveira, Ribeiro Rachel Melo, Bezerra Cícero Wellington Brito, Penha Rosiane Silva
Programa de Doutorado em Biotecnologia (RENORBIO), Universidade Federal do Maranhão (UFMA), São Luís, Brazil.
Curso de Biomedicina, Universidade Estácio de Sá, São Luís, Brazil.
AMB Express. 2022 Aug 4;12(1):102. doi: 10.1186/s13568-022-01433-7.
This study aims to produce, characterize, and assess the antimicrobial activity and cytotoxicity of polymer blends based on chitosan (CT) and fish collagen (COL) produced by different precipitation methods. Polymer blends were obtained in alkaline (NaOH), saline (NaCl), and alkaline/saline (NaOH/NaCl) solutions with different CT:COL concentration ratios (20:80, 50:50, and 80:20). The polymer blends were characterized by various physicochemical methods and subsequently evaluated in terms of their in vitro antimicrobial and cytotoxicity activity. In this study, the degree of chitosan deacetylation was 82%. The total hydroxyproline and collagen content in the fish matrix was 47.56 mg. g and 394.75 mg. g, respectively. The highest yield was 44% and was obtained for a CT:COL (80:20) blend prepared by precipitation in NaOH. High concentrations of hydroxyproline and collagen in the blends were observed when NaOH precipitation was used. Microbiological analysis revealed that the strains used in this work were sensitive to the biomaterial; this sensitivity was dose-dependent and increased with increasing chitosan concentration in the products. The biocompatibility test showed that the blends did not reduce the viability of fibroblast cells after 48 h of culture. An analysis of the microbiological activity of the all-polymer blends showed a decrease in the values of minimal inhibitory concentration (MIC) and minimal bactericidal concentrations (MBC) for S. aureus and P. aeruginosa. The blends showed biocompatibility with NIH-3T3 murine fibroblast cells and demonstrated their potential for use in biomedical applications such as wound healing, implants, and scaffolds.
本研究旨在制备、表征并评估基于壳聚糖（CT）和鱼胶原蛋白（COL）的聚合物共混物的抗菌活性和细胞毒性，这些聚合物共混物通过不同的沉淀方法制备。在具有不同CT:COL浓度比（20:80、50:50和80:20）的碱性（NaOH）、盐溶液（NaCl）和碱性/盐溶液（NaOH/NaCl）中获得聚合物共混物。通过各种物理化学方法对聚合物共混物进行表征，随后评估其体外抗菌和细胞毒性活性。在本研究中，壳聚糖的脱乙酰度为82%。鱼基质中总羟脯氨酸和胶原蛋白含量分别为47.56 mg/g和394.75 mg/g。最高产率为44%，是通过在NaOH中沉淀制备的CT:COL（80:20）共混物获得的。当使用NaOH沉淀时，共混物中观察到高浓度的羟脯氨酸和胶原蛋白。微生物分析表明，本研究中使用的菌株对该生物材料敏感；这种敏感性是剂量依赖性的，并且随着产品中壳聚糖浓度的增加而增加。生物相容性测试表明，共混物在培养48小时后不会降低成纤维细胞的活力。对所有聚合物共混物的微生物活性分析表明，金黄色葡萄球菌和铜绿假单胞菌的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）值降低。共混物与NIH-3T3小鼠成纤维细胞具有生物相容性，并证明了它们在伤口愈合、植入物和支架等生物医学应用中的潜在用途。
