College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, PR China.
College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, PR China.
Int J Biol Macromol. 2019 May 15;129:980-988. doi: 10.1016/j.ijbiomac.2019.02.079. Epub 2019 Feb 14.
A sulfonated chitosan (SCS) was prepared via nucleophilic substitution and characterized by Fourier transform infrared spectroscopy, H and C NMR spectra, gel permeation chromatography, elemental analysis, and thermo gravimetric analysis. The inhibition activities of bacterial adhesion and biofilm formation against E. coli and S. aureus of SCS were assessed in comparison with those of unmodified chitosan hydrochloride (WCS) which was commercially available. The metabolic activity and secretion of exopolysaccharide in biofilms of E. coli and S. aureus were significantly decreased after the treatment of SCS. Scanning electron microscopy and confocal laser scanning microscopy also demonstrated that SCS and WCS at 1MIC concentrations could obviously inhibit the formation of biofilm. Upon the experimental data obtained, it can be concluded that the alkylsulfonation of chitosan could significantly improve efficacy in killing biofilm-embedded bacteria, and the inhibition activities against biofilm formation of E. coli and S. aureus. The exploitation of SCS in this study is helpful to extend the understanding to an alternative to antibiotics and chemical preservatives in food and medicine fields.
通过亲核取代反应制备了磺化壳聚糖（SCS），并通过傅里叶变换红外光谱、H 和 C NMR 谱、凝胶渗透色谱、元素分析和热重分析对其进行了表征。与市售的未修饰壳聚糖盐酸盐（WCS）相比，评估了 SCS 对大肠杆菌和金黄色葡萄球菌的细菌粘附和生物膜形成的抑制活性。SCS 处理后，大肠杆菌和金黄色葡萄球菌生物膜中的代谢活性和胞外多糖分泌明显降低。扫描电子显微镜和共聚焦激光扫描显微镜也表明，SCS 和 WCS 在 1MIC 浓度下可以明显抑制生物膜的形成。根据实验数据可以得出结论，壳聚糖的烷基磺化可以显著提高杀死生物膜内细菌的功效，并抑制大肠杆菌和金黄色葡萄球菌的生物膜形成活性。本研究中 SCS 的开发有助于加深对食品和医药领域抗生素和化学防腐剂替代品的理解。
