药物抗性、毒力、疾病易感性以及宿主-鼠疟原虫相互作用决定因素的遗传图谱。
Genetic mapping of determinants in drug resistance, virulence, disease susceptibility, and interaction of host-rodent malaria parasites.
机构信息
Malaria Functional Genomics Section, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, MD, USA.
Malaria Functional Genomics Section, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, MD, USA.
出版信息
Parasitol Int. 2022 Dec;91:102637. doi: 10.1016/j.parint.2022.102637. Epub 2022 Aug 1.
Genetic mapping has been widely employed to search for genes linked to phenotypes/traits of interest. Because of the ease of maintaining rodent malaria parasites in laboratory mice, many genetic crosses of rodent malaria parasites have been performed to map the parasite genes contributing to malaria parasite development, drug resistance, host immune response, and disease pathogenesis. Drs. Richard Carter, David Walliker, and colleagues at the University of Edinburgh, UK, were the pioneers in developing the systems for genetic mapping of malaria parasite traits, including characterization of genetic markers to follow the inheritance and recombination of parasite chromosomes and performing the first genetic cross using rodent malaria parasites. Additionally, many genetic crosses of inbred mice have been performed to link mouse chromosomal loci to the susceptibility to malaria parasite infections. In this chapter, we review and discuss past and recent advances in genetic marker development, performing genetic crosses, and genetic mapping of both parasite and host genes. Genetic mappings using models of rodent malaria parasites and inbred mice have contributed greatly to our understanding of malaria, including parasite development within their hosts, mechanism of drug resistance, and host-parasite interaction.
遗传图谱绘制已被广泛用于寻找与感兴趣的表型/性状相关的基因。由于在实验室小鼠中维持鼠疟原虫较为容易，因此已经进行了许多鼠疟原虫的遗传杂交，以绘制有助于疟原虫发育、药物抗性、宿主免疫反应和疾病发病机制的寄生虫基因图谱。英国爱丁堡大学的 Richard Carter 博士、David Walliker 博士及其同事是开发疟原虫性状遗传图谱绘制系统的先驱，包括开发用于跟踪寄生虫染色体遗传和重组的遗传标记的特征，以及首次使用鼠疟原虫进行遗传杂交。此外，还进行了许多近交系小鼠的遗传杂交，以将小鼠染色体基因座与对疟原虫感染的敏感性联系起来。在本章中，我们回顾和讨论了过去和最近在遗传标记开发、遗传杂交以及寄生虫和宿主基因遗传图谱绘制方面的进展。使用鼠疟原虫和近交系小鼠模型进行的遗传图谱绘制极大地促进了我们对疟疾的理解，包括寄生虫在其宿主中的发育、药物抗性机制以及宿主-寄生虫相互作用。
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