Gao Jiamin, Wang Nian, Jia Jichun, Xie Jiatao, Jiang Daohong, Zhang Lili
College of Life Science, Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production and Construction Corps, Tarim University, Alar, 843300, China.
State Key Laboratory of Agricultural Microbiology, The Provincial Key Lab of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
Arch Virol. 2022 Nov;167(11):2387-2390. doi: 10.1007/s00705-022-05524-5. Epub 2022 Aug 4.
A new positive-sense single-stranded RNA (+ssRNA) mycovirus, Verticillium dahliae magoulivirus 1 (VdMoV1), was isolated from two strains (2-19 and XLZ70) of Verticillium dahliae. The complete genome of VdMoV1 is 2303 nucleotides (nt) in length and has a large open reading frame (nt positions from 61 to 1938) encoding an RNA-dependent RNA polymerase (RdRp). A multiple sequence alignment indicated that the central region of the RdRp encoded by VdMoV1 contains eight typical viral RdRp motifs. BLASTp analysis demonstrated that VdMoV1 has the highest sequence identity (86.88%) to Bremia lactucae associated ourmia-like virus 2 (BlaOLV2). Phylogenetic analysis revealed that VdMoV1 is a new member of the genus Magoulivirus. As far as we know, VdMoV1 is the first reported member of the family Botourmiaviridae infecting V. dahliae.
一种新的正链单链RNA（+ssRNA）真菌病毒，大丽轮枝菌马古病毒1（VdMoV1），从大丽轮枝菌的两个菌株（2-19和XLZ70）中分离得到。VdMoV1的完整基因组长度为2303个核苷酸（nt），有一个大的开放阅读框（核苷酸位置从61到1938），编码一种RNA依赖的RNA聚合酶（RdRp）。多重序列比对表明，VdMoV1编码的RdRp的中心区域包含八个典型的病毒RdRp基序。BLASTp分析表明，VdMoV1与莴苣盘梗霉相关的奥尔米样病毒2（BlaOLV2）具有最高的序列同一性（86.88%）。系统发育分析表明，VdMoV1是马古病毒属的一个新成员。据我们所知，VdMoV1是首次报道的感染大丽轮枝菌的布托奥尔米病毒科成员。
