从植物病原菌核盘菌中分离到的一种新型布托病毒的特性。
Characterization of a novel botoulivirus isolated from the phytopathogenic fungus Sclerotinia sclerotiorum.
机构信息
State Key Laboratory of Agricultural Microbiology, The Provincial Key Lab of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
出版信息
Arch Virol. 2021 Oct;166(10):2859-2863. doi: 10.1007/s00705-021-05168-x. Epub 2021 Jul 22.
Sclerotinia sclerotiorum ourmiavirus 17 (SsOV17) was isolated from the hypovirulent strain GF3 of Sclerotinia sclerotiorum. The genome of SsOV17 is 2,802 nt in length and contains a single long open reading frame (ORF) flanked by a short structured 5'-untranslated region (5'-UTR) (28 nt) and a long 3'-UTR (788 nt), respectively. The ORF encodes a protein with 663 amino acids and a predicted molecular mass of 75.0 kDa. A BLASTp search indicated that the protein encoded by SsOV17 is closely related to the putative RNA-dependent RNA polymerase (RdRp) of Sclerotinia sclerotiorum ourmiavirus 13 (71% identity). A multiple sequence alignment indicated that eight conserved amino acid motifs were present in the RdRp conserved region of SsOV17. Phylogenetic analysis demonstrated that SsOV17 clustered with members of the genus Botoulivirus.
奥氏核盘菌坏死病毒 17 号（SsOV17）是从核盘菌弱毒株 GF3 中分离出来的。SsOV17 的基因组长 2802 个核苷酸，包含一个单一的长开放阅读框（ORF），两侧分别是一个短的结构 5'非翻译区（5'-UTR）（28 个核苷酸）和一个长的 3'非翻译区（788 个核苷酸）。ORF 编码一个具有 663 个氨基酸和预测分子量为 75.0 kDa 的蛋白质。BLASTp 搜索表明，SsOV17 编码的蛋白与奥氏核盘菌坏死病毒 13 的推定 RNA 依赖性 RNA 聚合酶（RdRp）密切相关（71%的同一性）。多重序列比对表明，SsOV17 的 RdRp 保守区存在 8 个保守的氨基酸基序。系统发育分析表明，SsOV17 与 Botoulivirus 属的成员聚类在一起。
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