Johnsson A, Kjeldstad B, Melø T B
Arch Dermatol Res. 1987;279(3):190-3. doi: 10.1007/BF00413256.
Fluorescence studies were performed on the extrusions from pilosebaceous follicles. Pressure extractions produced follicle samples which showed fluorescence under Wood's light. The samples were then analysed in a fluorometer giving corrected excitation spectra. The structured emission spectra achieved were interpreted as being due to porphyrins produced by Propionibacterium acnes (P. acnes). Details in the spectra showed close resemblance to spectra from cultured P. acnes cells. The emission spectra showed distinct features in all the four subjects investigated (who were different with respect to age, sex, follicle sampling area, and tendency to acne) and dominant peaks due to at least three porphyrins were found. The concentrations of these porphyrins vary from case to case. Excitation spectra were recorded and supported the assumption that the fluorescent emission was partly due to coproporphyrins and metalloporphyrins in the samples. Free protoporphyrins did not seem to be present in the extrusions. The excitation spectra, in particular, vary from person to person but seem to be constant over time in one and the same subject.
对毛囊皮脂腺的挤出物进行了荧光研究。压力提取产生的毛囊样本在伍德灯下显示出荧光。然后在荧光计中对样本进行分析，得出校正后的激发光谱。所获得的结构化发射光谱被解释为是由痤疮丙酸杆菌（P. acnes）产生的卟啉所致。光谱细节显示与培养的痤疮丙酸杆菌细胞的光谱非常相似。在所研究的所有四名受试者（他们在年龄、性别、毛囊取样区域和痤疮倾向方面各不相同）中，发射光谱都显示出明显特征，并且发现了至少三种卟啉的主峰。这些卟啉的浓度因病例而异。记录了激发光谱，并支持了荧光发射部分归因于样本中的粪卟啉和金属卟啉这一假设。挤出物中似乎不存在游离原卟啉。特别是激发光谱因人而异，但在同一个体中似乎随时间保持恒定。
