Puhvel S M, Reisner R M, Amirian D A
J Invest Dermatol. 1975 Dec;65(6):525-31. doi: 10.1111/1523-1747.ep12610239.
A technique for quantitating bacteria in isolated pilosebaceous follicles is described. This involves microdissection of the follicles from biopsies of skin, using the method of chemical pretreatment of skin to facilitate the separation of the epidermis and epidermal appendages from the dermis. The aerobic cocci and anaerobic diphtheroids in pilosebaceous follicles in 66 biopsies of scalp and 48 biopsies of skin of the upper back were quantitated using this technique. On the back, aerobic staphylococci were very sparse in normal follicles, indicating that their primary habitat on the skin must be on the skin surface rather than within follicles. Of 138 isolated follicles from skin of the upper back, 94 contained no aerobic cocci. Anaerobic organisms were present in high numbers within normal follicles. The geometric mean density of anaerobes in 138 isolated follicles from skin of the upper back was 3.8 X 10(4) diphtheroids per follicle. Eighty-eight follicles contained more than 10(4) anaerobic diphtheroids. Using data from scalp biopsies we found that there was a correlation between the weight of sebaceous glands and the density of anaerobes within the follicles attached to these glands (coefficient of correlation = 0.6).
本文描述了一种定量分析孤立毛囊中细菌的技术。该技术包括从皮肤活检组织中显微解剖毛囊，采用对皮肤进行化学预处理的方法，以促进表皮和表皮附属器与真皮的分离。使用该技术对66例头皮活检组织和48例上背部皮肤活检组织中的毛囊内需氧球菌和厌氧类白喉杆菌进行了定量分析。在上背部，正常毛囊中的需氧葡萄球菌非常稀少，这表明它们在皮肤上的主要栖息地一定是在皮肤表面而非毛囊内。在上背部皮肤的138个孤立毛囊中，94个没有需氧球菌。正常毛囊内存在大量厌氧菌。在上背部皮肤的138个孤立毛囊中，厌氧菌的几何平均密度为每个毛囊3.8×10⁴个类白喉杆菌。88个毛囊中含有超过10⁴个厌氧类白喉杆菌。利用头皮活检组织的数据，我们发现皮脂腺重量与附着于这些腺体的毛囊内厌氧菌密度之间存在相关性（相关系数 = 0.6）。
