Sornchuer Phornphan, Saninjuk Kritsakorn, Prathaphan Parisa, Tiengtip Rattana, Wattanaphansak Suphot
Microbiology and Immunology, Department of Preclinical Science, Faculty of Medicine, Thammasat University, Klongluang 12120, Pathum Thani, Thailand.
Thammasat University Research Unit in Nutraceuticals and Food Safety, Faculty of Medicine, Thammasat University, Klongluang 12120, Pathum Thani, Thailand.
Microorganisms. 2022 Jan 23;10(2):252. doi: 10.3390/microorganisms10020252.
Members of the group are considered to be foodborne pathogens commonly associated with diarrheal and emetic gastrointestinal syndromes. Biofilm formation is a major virulence determinant of various pathogenic bacteria, including the strains, since it can protect the bacteria against antimicrobial agents and the host immune response. Moreover, a biofilm allows the exchange of genetic material, such as antimicrobial resistance genes, among the different bacterial strains inside the matrix. The aim of the current study was to genotypically and phenotypically characterize sp. B87, a strain that was isolated from food and which exhibited strong biofilm-forming capacity. Based on the analysis of the phylogenetic relationship, the isolate was phylogenetically mapped close to . Antimicrobial susceptibility testing revealed that the isolate was resistant to tetracycline and β-lactam antimicrobial agents, which corresponded with the genotypic characterization using the whole-genome analysis. The genome of sp. B87 carried the three-component non-hemolytic enterotoxin (NHE), which is a type of enterotoxin that causes diarrheal symptoms. In addition, the genome also contained several genes that participate in biofilm formation, including the operon. These findings expand our understanding of antimicrobial resistance and virulence in species based on the link between genotypic and phenotypic characterization.
该菌属的成员被认为是通常与腹泻和呕吐性胃肠综合征相关的食源性病原体。生物膜形成是包括该菌株在内的各种病原菌的主要毒力决定因素，因为它可以保护细菌免受抗菌剂和宿主免疫反应的影响。此外，生物膜允许基质内不同细菌菌株之间交换遗传物质，如抗微生物耐药基因。本研究的目的是对从食物中分离出的具有很强生物膜形成能力的某菌属B87菌株进行基因分型和表型特征分析。基于系统发育关系分析，该分离株在系统发育上被定位在接近某菌属的位置。抗菌药敏试验表明，该分离株对四环素和β-内酰胺类抗菌剂耐药，这与使用全基因组分析的基因分型结果一致。某菌属B87菌株的基因组携带三组分非溶血性肠毒素（NHE），这是一种引起腹泻症状的肠毒素类型。此外，该基因组还包含几个参与生物膜形成的基因，包括某操纵子。这些发现基于基因分型和表型特征之间的联系，扩展了我们对某菌属物种中抗菌耐药性和毒力的理解。
