Yu Shubo, Yu Pengfei, Wang Juan, Li Chun, Guo Hui, Liu Chengcheng, Kong Li, Yu Leyi, Wu Shi, Lei Tao, Chen Moutong, Zeng Haiyan, Pang Rui, Zhang Youxiong, Wei Xianhu, Zhang Jumei, Wu Qingping, Ding Yu
State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, China.
Department of Food Science and Technology, Institute of Food Safety and Nutrition, Jinan University, Guangzhou, China.
Front Microbiol. 2020 Jan 15;10:3043. doi: 10.3389/fmicb.2019.03043. eCollection 2019.
is widely distributed in different food products and can cause a variety of symptoms associated with food poisoning. Since ready-to-eat (RTE) foods are not commonly sterilized by heat treatment before consumption, contamination may cause severe food safety problems. In this study, we investigated the prevalence of in RTE food samples from different regions of China and evaluated the levels of bacterial contamination, antibiotic resistance, virulence gene distribution, and genetic polymorphisms of these isolates. Of the tested retail RTE foods, 35% were positive for , with 39 and 83% of the isolated strains harboring the enterotoxin-encoding and gene clusters, respectively. The gene was detected in all . strains. The gene was present in 68% of isolates, but only 7% harbored the emetic toxin-encoding gene . Antimicrobial susceptibility testing revealed that the majority of the isolates were resistant not only to most β-lactam antibiotics, but also to rifamycin. Multilocus sequence typing (MLST) revealed that the 368 isolates belonged to 192 different sequence types (STs) including 93 new STs, the most prevalent of which was ST26. Collectively, our study indicates the prevalence, bacterial contamination levels, and biological characteristics of isolated from RTE foods in China and demonstrates the potential hazards of in RTE foods.
广泛分布于不同食品中,可引发多种与食物中毒相关的症状。由于即食(RTE)食品在食用前通常未经热处理杀菌,污染可能导致严重的食品安全问题。在本研究中,我们调查了中国不同地区即食食品样本中的 流行情况,并评估了这些分离株的细菌污染水平、抗生素耐药性、毒力基因分布及基因多态性。在所检测的零售即食食品中,35% 对 呈阳性,分离菌株中分别有39% 和83% 携带编码肠毒素的 和 基因簇。所有 菌株均检测到 基因。68% 的分离株存在 基因,但只有7% 携带编码呕吐毒素的基因 。抗菌药敏试验表明,大多数分离株不仅对大多数β-内酰胺类抗生素耐药,而且对利福霉素耐药。多位点序列分型(MLST)显示,368株分离株属于192种不同的序列类型(STs),包括93种新的STs,其中最常见的是ST26。总体而言,我们的研究表明了从中国即食食品中分离出的 的流行情况、细菌污染水平及生物学特性,并证明了即食食品中 的潜在危害。