Guangdong Provincial Key Laboratory of Applied Botany, Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, Core Botanical Gardens, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China; College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China.
Guangdong Provincial Key Laboratory of Applied Botany, Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, Core Botanical Gardens, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
Carbohydr Polym. 2022 Feb 15;278:118939. doi: 10.1016/j.carbpol.2021.118939. Epub 2021 Nov 26.
Ganoderma lucidum spore serves as a well-known immunomodulatory functional food in Asia. The polysaccharides in G. lucidum spore are responsible for the claimed immunomodulatory activity. However, the structural information of polysaccharides remains unclear. In this work, the leading water-soluble polysaccharide in G. lucidum spore (GLSP-I) with a molecular weight of 128.0 kDa was isolated and purified. The monosaccharide composition analysed by gas chromatography indicated that GLSP-I was a glucan. Three side chains, including Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc, were identified by UPLC-MS/MS. The structural characteristics were further identified by NMR spectra. The results indicated that the backbone of GLSP-I was (1 → 3)-β-D-glucan, with side chains linking at O-6. The proposed structure was drawn as below. The immunomodulatory activity assay indicated that GLSP-I could activate macrophages in a dose-dependent manner.
灵芝孢子粉作为亚洲著名的免疫调节功能性食品。灵芝孢子粉中的多糖是其声称的免疫调节活性的主要成分。然而，多糖的结构信息仍不清楚。在这项工作中，从灵芝孢子粉中分离纯化出一种分子量为 128.0 kDa 的主要水溶性多糖(GLSP-I)。气相色谱分析的单糖组成表明 GLSP-I 是一种葡聚糖。通过 UPLC-MS/MS 鉴定出三种侧链，分别为 Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc、Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc 和 Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc。通过 NMR 光谱进一步确定了其结构特征。结果表明，GLSP-I 的主链为(1 → 3)-β-D-葡聚糖，侧链连接在 O-6 位。提出的结构如下所示。免疫调节活性测定表明，GLSP-I 可剂量依赖性地激活巨噬细胞。
