Maïga Hamidou, Bakhoum Mame Thierno, Mamai Wadaka, Diouf Gorgui, Bimbilé Somda Nanwintoum Séverin, Wallner Thomas, Martina Claudia, Kotla Simran Singh, Masso Odet Bueno, Yamada Hanano, Sow Bazoumana B D, Fall Assane Gueye, Bouyer Jeremy
Insect Pest Control Laboratory, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, IAEA Laboratories, Seibersdorf, P.O. Box 100, A-1400 Vienna, Austria.
Institut de Recherche en Sciences de la Santé/Direction Régionale de l'Ouest (IRSS-DRO), Bobo-Dioulasso BP 2779, Burkina Faso.
Insects. 2023 Feb 18;14(2):207. doi: 10.3390/insects14020207.
Pilot programs of the sterile insect technique (SIT) against may rely on importing significant and consistent numbers of high-quality sterile males from a distant mass rearing factory. As such, long-distance mass transport of sterile males may contribute to meet this requirement if their survival and quality are not compromised. This study therefore aimed to develop and assess a novel method for long-distance shipments of sterile male mosquitoes from the laboratory to the field. Different types of mosquito compaction boxes in addition to a simulation of the transport of marked and unmarked sterile males were assessed in terms of survival rates/recovery rates, flight ability and morphological damage to the mosquitoes. The novel mass transport protocol allowed long-distance shipments of sterile male mosquitoes for up to four days with a nonsignificant impact on survival (>90% for 48 h of transport and between 50 and 70% for 96 h depending on the type of mosquito compaction box), flight ability, and damage. In addition, a one-day recovery period for transported mosquitoes post-transport increased the escaping ability of sterile males by more than 20%. This novel system for the long-distance mass transport of mosquitoes may therefore be used to ship sterile males worldwide for journeys of two to four days. This study demonstrated that the protocol can be used for the standard mass transport of marked or unmarked chilled mosquitoes required for the SIT or other related genetic control programs.
针对[未提及具体对象]的昆虫不育技术（SIT）试点项目可能依赖于从遥远的大规模饲养工厂进口大量且稳定的高质量不育雄虫。因此，如果不育雄虫的存活率和质量不受影响，那么远距离大规模运输不育雄虫可能有助于满足这一需求。因此，本研究旨在开发并评估一种将不育雄蚊从实验室远距离运输到野外的新方法。除了模拟标记和未标记不育雄蚊的运输外，还对不同类型的蚊虫压缩箱在存活率/回收率、飞行能力以及蚊虫形态损伤方面进行了评估。这种新的大规模运输方案允许不育雄蚊进行长达四天的远距离运输，且对其存活率（运输48小时后>90%，运输96小时后根据蚊虫压缩箱类型在50%至70%之间）、飞行能力和损伤影响不显著。此外，运输后的蚊虫有一天的恢复期，可使不育雄蚊的逃逸能力提高20%以上。因此，这种用于蚊虫远距离大规模运输的新系统可用于在全球范围内运输不育雄蚊，运输时长为两到四天。本研究表明，该方案可用于昆虫不育技术或其他相关遗传控制项目所需的标记或未标记冷藏蚊虫的标准大规模运输。
