Espion D, de Henau S, Letellier C, Wemers C D, Brasseur R, Young J F, Gross M, Rosenberg M, Meulemans G, Burny A
Arch Virol. 1987;95(1-2):79-95. doi: 10.1007/BF01311336.
A cDNA library was constructed with poly(A)+-mRNAs from NDV-Italien infected BHK-21 cells. A clone, that hybridized to the F gene mRNA, was sequenced. A long open reading frame encodes for a protein of 553 amino acids, with a calculated molecular weight of 59,153, consisting of twelve cysteine residues and six potential glycosylation sites. The protein sequence contains a hydrophobic region at the N-terminus of F1 and a presumptive long transmembrane fragment near the C-terminus. Comparison of the F proteins from NDV strains Italien and Australia-Victoria shows that the sequences are very similar, with conservation of most cysteine residues and of the potential glycosylation sites. The F coding sequence was inserted into the genome of vaccinia virus under the control of vaccinia P7.5 transcriptional regulatory sequences. Expression of F protein was demonstrated by indirect immunofluorescence with five anti-F monoclonal antibodies known to react with conformational epitopes.
用新城疫病毒意大利株（NDV-Italien）感染的BHK-21细胞的聚腺苷酸加尾（poly(A)+）mRNA构建了一个cDNA文库。对一个与F基因mRNA杂交的克隆进行了测序。一个长开放阅读框编码一个由553个氨基酸组成的蛋白质，计算分子量为59,153，该蛋白质由12个半胱氨酸残基和6个潜在的糖基化位点组成。蛋白质序列在F1的N端含有一个疏水区域，在C端附近有一个推测的长跨膜片段。新城疫病毒意大利株和澳大利亚维多利亚株的F蛋白序列比较表明，它们非常相似，大多数半胱氨酸残基和潜在的糖基化位点都得到了保留。F编码序列在痘苗病毒P7.5转录调控序列的控制下被插入痘苗病毒基因组。用已知能与构象表位反应的5种抗F单克隆抗体通过间接免疫荧光法证实了F蛋白的表达。
