McGinnes L W, Morrison T G
Virus Res. 1986 Sep;5(4):343-56. doi: 10.1016/0168-1702(86)90028-6.
The nucleotide sequence of cloned cDNA copies of the mRNA encoding the Newcastle disease virus fusion protein was determined. A single open reading frame in the sequence encodes a hydrophobic protein of 553 amino acids with a calculated molecular weight of 58 978. The previously determined protein sequence of the amino terminus of the F1 (Richardson, G.D. et al. (1980) Virology 105, 205-222) was located within the predicted protein sequence. The predicted protein sequence contains a hydrophobic stretch of 29 amino acids near the carboxy terminal end and likely represents the membrane spanning region of the protein. The F2 portion of the sequence contains one glycosylation site while F1 contains four which are potentially used. The predicted sequence contains 13 cysteine residues. Comparison of the NDV fusion protein sequence with three other paramyxovirus fusion protein sequences reveals little homology common to all four viruses except for the amino terminus of the F1 proteins. However, the positions of the cysteine residues within the sequence are conserved, particularly among the members of the paramyxovirus subgroup, suggesting the importance of disulfide bond formation in the conformation of paramyxovirus fusion proteins.
测定了编码新城疫病毒融合蛋白的mRNA的克隆cDNA拷贝的核苷酸序列。该序列中的一个单一开放阅读框编码一个由553个氨基酸组成的疏水蛋白，计算分子量为58978。先前确定的F1氨基末端的蛋白质序列（Richardson, G.D.等人，(1980) Virology 105, 205 - 222）位于预测的蛋白质序列内。预测的蛋白质序列在羧基末端附近包含一段由29个氨基酸组成的疏水区域，可能代表该蛋白质的跨膜区域。序列的F2部分包含一个糖基化位点，而F1包含四个可能被利用的糖基化位点。预测序列包含13个半胱氨酸残基。将新城疫病毒融合蛋白序列与其他三种副粘病毒融合蛋白序列进行比较，发现除了F1蛋白的氨基末端外，这四种病毒几乎没有共同的同源性。然而，序列中半胱氨酸残基的位置是保守的，特别是在副粘病毒亚组的成员之间，这表明二硫键形成在副粘病毒融合蛋白构象中的重要性。
