Charlemagne D, Schwartz K, Bernard S, Paraf A
J Immunol Methods. 1979;29(2):145-53. doi: 10.1016/0022-1759(79)90064-4.
Using the ELISA technique we have been able to quantify antibodies directed against actin and to follow the kinetics of antibody production. Specific anti-actin antisera have been raised in rabbits by immunization with chemically modified white muscle rabbit actin. Two or three dinitrophenyl groups linked per actin molecule were sufficient to break natural tolerance, while linkage of three phosphorylcholine groups to actin was not.
运用酶联免疫吸附测定（ELISA）技术，我们得以对针对肌动蛋白的抗体进行定量，并追踪抗体产生的动力学过程。通过用化学修饰的白色肌肉兔肌动蛋白免疫兔子，制备出了特异性抗肌动蛋白抗血清。每个肌动蛋白分子连接两到三个二硝基苯基基团足以打破天然耐受性，而三个磷酰胆碱基团与肌动蛋白的连接则无法做到这一点。
