Key Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, Hainan Aquaculture Breeding Engineering Research Center, College of Marine Sciences, Hainan University, Haikou, Hainan, 570228, China.
Key Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, Hainan Aquaculture Breeding Engineering Research Center, College of Marine Sciences, Hainan University, Haikou, Hainan, 570228, China.
Fish Shellfish Immunol. 2022 Nov;130:141-154. doi: 10.1016/j.fsi.2022.07.048. Epub 2022 Aug 3.
To understand the regulatory mechanism of Janus kinase/Signal Transducers and Activators of Transcription (JAK/STAT) signaling pathway on the immune system of the Pacific white shrimp, Litopenaeus vannamei, RNA interference technique was used to investigate the effects of JAK/STAT signaling pathway on the immune response of hemocyte in Litopenaeus vannamei stimulated by lipopolysaccharide (LPS). The results showed that 1) after 6 h of LPS stimulation, the expression levels of immune genes in hemocyte were significantly up-regulated (P < 0.05), the immune defense ability (hemocyte number, phagocytosis rate, hemagglutination activity, bacteriolytic activity, antibacterial activity, prophenoloxidase system activity) and the hemocyte antioxidant ability were significantly higher than the control group, especially at 12 h. 2) After 48 h of STAT gene interference, the expression levels of immune genes in hemocytes were significantly down-regulated, and the immune defense ability (hemocyte count, phagocytosis rate, plasma agglutination activity, lysozyme activity, antibacterial activity, proPO system activity) and the antioxidant ability were reduced and significantly lower than control. Concurrently, after LPS stimulation, the immune indexes were significantly up-regulated at 12 h to the maximum but was still lower the undisturbed LPS group. These results indicate that JAK/STAT signaling pathway is involved in the immune regulation mechanism of L. vannamei against LPS stimulation through positive regulation of cellular immune and humoral immune. These results provide a basis for further research on the role and status of JAK/STAT signaling pathway in the immune defense of crustaceans.
为了理解 Janus 激酶/信号转导与转录激活因子（JAK/STAT）信号通路对凡纳滨对虾免疫系统的调控机制，采用 RNA 干扰技术研究了 JAK/STAT 信号通路对脂多糖（LPS）刺激凡纳滨对虾血细胞免疫应答的影响。结果显示：1）LPS 刺激 6 h 后，血细胞中免疫基因的表达水平显著上调（P<0.05），血细胞的免疫防御能力（血细胞数量、吞噬率、血淋巴凝集活性、溶菌活性、抗菌活性、酚氧化酶原系统活性）和抗氧化能力显著高于对照组，尤其是 12 h；2）STAT 基因干扰 48 h 后，血细胞中免疫基因的表达水平显著下调，血细胞数量、吞噬率、血浆凝集活性、溶菌酶活性、抗菌活性、酚氧化酶原系统活性和抗氧化能力均降低，且显著低于对照组。同时，LPS 刺激后，免疫指标在 12 h 时显著上调至最大值，但仍低于未受干扰的 LPS 组。这些结果表明，JAK/STAT 信号通路通过对细胞免疫和体液免疫的正调控，参与了凡纳滨对虾对 LPS 刺激的免疫调节机制。这些结果为进一步研究 JAK/STAT 信号通路在甲壳动物免疫防御中的作用和地位提供了依据。
