Chen Hui, Zhu Ying, Zhang Ya-Lun, Zeng Meng-Nan, Cao Yan-Gang, Sun Pan-Ting, Cao Bing, Du Kun, Zhao Xuan, Wang Xiao-Wei, Zheng Xiao-Ke, Feng Wei-Sheng
School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, 450046, PR China; Co-Construction Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases by Henan & Education Ministry of PR China, Zhengzhou, 450046, PR China; NMPA Key Laboratory for Quality Control of Traditional Chinese Medicine (Chinese Materia Medica and prepared slices), Zhengzhou, 450018, PR China.
School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, 450046, PR China.
Phytochemistry. 2022 Nov;203:113336. doi: 10.1016/j.phytochem.2022.113336. Epub 2022 Aug 4.
Four undescribed neolignans and three undescribed amide alkaloids, along with twelve known compounds, were isolated from the stems of Piper kadsura (Choisy) Ohwi. The structures of the new compounds were determined by spectroscopic analysis, quantum-chemical calculations, and Mo(OAc)-induced ECD analysis. The neuroprotective effects of these compounds against Aβ-induced cell damage in PC12 cells were investigated, and eight compounds exhibited significant neuroprotective effects against Aβ-induced PC12 cell damage, with the EC values of 3.06-29.3 μM. Three of these compounds were selected for further experiments, and they appear to reduce apoptosis and enhance autophagy against Aβ-induced PC12 cell damage.
从海风藤（Piper kadsura (Choisy) Ohwi）的茎中分离出了4个未描述的新木脂素和3个未描述的酰胺生物碱，以及12个已知化合物。通过光谱分析、量子化学计算和钼（乙酸）诱导的ECD分析确定了新化合物的结构。研究了这些化合物对PC12细胞中Aβ诱导的细胞损伤的神经保护作用，8种化合物对Aβ诱导的PC12细胞损伤表现出显著的神经保护作用，其EC值为3.06 - 29.3 μM。选择其中3种化合物进行进一步实验，它们似乎能减少Aβ诱导的PC12细胞损伤中的细胞凋亡并增强自噬。
