Micheli V, Sestini S, Rocchigiani M, Pescaglini M, Ricci C
Biomed Biochim Acta. 1987;46(2-3):S268-72.
The regulation of erythrocyte synthesis of nicotinate and adenine nucleotides has been investigated. Some effectors of the two committed enzymes, nicotinate- and adenine phosphoribosyltransferases, have been identified on crude lysates and on partially purified preparations of the former. Enzyme characteristics have been correlated with the nucleotide synthesis achieved in intact cells incubated in suitable mediums containing (14-C)-nicotinate or adenine. Inorganic phosphate, Mg ions and adenine nucleotides proved to be important effectors of pyridine synthesis, whose products, in turn, do not influence adenine nucleotide production. The production of pyridine nucleotides is little lower than that of adenine nucleotides in intact cells, even if adenine phosphoribosyl-transferase activity appears to be much more limited, inside the cell, than nicotinate phosphoribosyltransferase.
对红细胞中烟酸和腺嘌呤核苷酸合成的调控进行了研究。已在粗裂解物和部分纯化的烟酰胺磷酸核糖转移酶制剂上鉴定出这两种关键酶（烟酸磷酸核糖转移酶和腺嘌呤磷酸核糖转移酶）的一些效应物。酶的特性已与在含有（14-C）-烟酸或腺嘌呤的合适培养基中孵育的完整细胞中实现的核苷酸合成相关联。无机磷酸盐、镁离子和腺嘌呤核苷酸被证明是吡啶合成的重要效应物，而其产物反过来并不影响腺嘌呤核苷酸的产生。即使在细胞内腺嘌呤磷酸核糖转移酶的活性似乎比烟酸磷酸核糖转移酶受到更多限制，但完整细胞中吡啶核苷酸的产生略低于腺嘌呤核苷酸的产生。
