Center of Deep Sea Research & CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China.
Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, 266071, China.
Sci Data. 2022 Aug 6;9(1):480. doi: 10.1038/s41597-022-01586-x.
Cold seep microbial communities are fascinating ecosystems on Earth which provide unique models for understanding the living strategies in deep-sea distinct environments. In this study, 23 metagenomes were generated from samples collected in the Site-F cold seep field in South China Sea, including the sea water closely above the invertebrate communities, the cold seep fluids, the fluids under the invertebrate communities and the sediment column around the seep vent. By binning tools, we retrieved a total of 768 metagenome assembled genome (MAGs) that were estimated to be >60% complete. Of the MAGs, 61 were estimated to be >90% complete, while an additional 105 were >80% complete. Phylogenomic analysis revealed 597 bacterial and 171 archaeal MAGs, of which nearly all were distantly related to known cultivated isolates. In the 768 MAGs, the abundant Bacteria in phylum level included Proteobacteria, Desulfobacterota, Bacteroidota, Patescibacteria and Chloroflexota, while the abundant Archaea included Asgardarchaeota, Thermoplasmatota, and Thermoproteota. These results provide a dataset available for further interrogation of deep-sea microbial ecology.
冷泉微生物群落是地球上引人入胜的生态系统，为深入了解深海特殊环境中的生存策略提供了独特的模型。在这项研究中，从南海冷泉场站点 F 采集的样本中生成了 23 个宏基因组，包括位于无脊椎动物群落上方的海水、冷泉流体、无脊椎动物群落下方的流体以及冷泉喷口周围的沉积柱。通过 binning 工具，我们总共检索到了 768 个宏基因组组装基因组（MAG），估计其完整度超过 60%。其中，61 个估计完整度超过 90%，另有 105 个估计完整度超过 80%。系统发育基因组分析揭示了 597 个细菌和 171 个古菌 MAG，其中几乎所有 MAG 与已知的培养分离物都有较远的亲缘关系。在 768 个 MAG 中，门水平的优势细菌包括变形菌门、脱硫杆菌门、拟杆菌门、Patescibacteria 和绿弯菌门，而优势古菌包括阿斯加德古菌门、热原体门和热变形菌门。这些结果提供了一个可用于进一步探究深海微生物生态学的数据集。
