Li Yuping, Zhuang Feng, Zeng Jiangyi, Yang Chao, Li Yongqing, Luo Ming, Wang Ying
Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China.
College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China.
J Cell Biochem. 2022 Nov;123(11):1780-1792. doi: 10.1002/jcb.30315. Epub 2022 Aug 7.
Glycyrrhiza inflata Bat. is a type of abiotic-stress-resistant plant with extremely high medicinal value. Histone demethylases (HDMTs) have been known to play crucial roles in the regulation of abiotic stress response. However, the molecular functions of HDMTs has not been studied in G. inflata. Here we identified 34 GiHDMT genes in G. inflata, which could be divided into the 6 groups through phylogenetic analysis. We further found that the gene structure and conserved protein motifs exhibit high conservation in each group of GiHDMT genes. Various abiotic-stress-related elements are detected in GiHDMT promoters, especially for the light-responsive element and abscisic acid-responsive element. Collinearity analysis indicated that segmental duplication contributed to the expansion of the GiHDMT family in licorice. Subcellular localization analysis revealed that green fluorescent protein-tagged GiHDMT2 and GiHDMT18 were predominantly localized in the nucleus, whereas GiHDMT1 were found in both the cytoplasm and nucleus. Real-time quantitative polymerase chain reaction showed that GiHDMTs presented differential expression patterns across different tissues. Moreover, changes in transcription level of GiHDMTs under abiotic stress indicate the potential role of GiHDMTs in the stress response in licorice. Finally, we found the histone methylation levels probably mediated by GiHDMT genes are changed with the treatment of NaCl and Na CO . Our study will lay the foundation for future research on the regulatory roles of GiHDMT genes in the environmental stress.
胀果甘草是一种具有极高药用价值的抗非生物胁迫植物。已知组蛋白去甲基化酶（HDMTs）在非生物胁迫反应的调控中起关键作用。然而，尚未在胀果甘草中研究HDMTs的分子功能。在此，我们在胀果甘草中鉴定出34个GiHDMT基因，通过系统发育分析可将其分为6组。我们进一步发现，GiHDMT基因每组中的基因结构和保守蛋白基序具有高度保守性。在GiHDMT启动子中检测到各种与非生物胁迫相关的元件，尤其是光响应元件和脱落酸响应元件。共线性分析表明，片段重复促进了甘草中GiHDMT家族的扩张。亚细胞定位分析显示，绿色荧光蛋白标记的GiHDMT2和GiHDMT18主要定位于细胞核，而GiHDMT1在细胞质和细胞核中均有发现。实时定量聚合酶链反应表明，GiHDMTs在不同组织中呈现差异表达模式。此外，非生物胁迫下GiHDMTs转录水平的变化表明GiHDMTs在甘草胁迫反应中的潜在作用。最后，我们发现经NaCl和Na₂CO₃处理后，可能由GiHDMT基因介导的组蛋白甲基化水平发生了变化。我们的研究将为未来研究GiHDMT基因在环境胁迫中的调控作用奠定基础。
