Guangdong Provincial Key Laboratory of Applied Botany, Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China.
Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou, 510631, China.
BMC Plant Biol. 2018 Oct 11;18(1):226. doi: 10.1186/s12870-018-1454-7.
Histone deacetylases (HDACs) function as key epigenetic factors in repressing the expression of genes in multiple aspects of plant growth, development and plant response to abiotic or biotic stresses. To date, the molecular function of HDACs is well described in Arabidopsis thaliana, but no systematic analysis of this gene family in soybean (Glycine max) has been reported.
In this study, 28 HDAC genes from soybean genome were identified, which were asymmetrically distributed on 12 chromosomes. Phylogenetic analysis demonstrated that GmHDACs fall into three major groups previously named RPD3/HDA1, SIR2, and HD2. Subcellular localization analysis revealed that YFP-tagged GmSRT4, GmHDT2 and GmHDT4 were predominantly localized in the nucleus, whereas GmHDA6, GmHDA13, GmHDA14 and GmHDA16 were found in both the cytoplasm and nucleus. Real-time quantitative PCR showed that GmHDA6, GmHDA13, GmHDA14, GmHDA16 and GmHDT4 were broadly expressed across plant tissues, while GmHDA8, GmSRT2, GmSRT4 and GmHDT2 showed differential expression across various tissues. Interestingly, we measured differential changes in GmHDACs transcripts accumulation in response to several abiotic cues, indicating that these epigenetic modifiers could potentially be part of a dynamic transcriptional response to stress in soybean. Finally, we show that the levels of histone marks previously reported to be associated with plant HDACs are modulated by cold and heat in this legume.
We have identified and classified 28 HDAC genes in soybean. Our data provides insights into the evolution of the HDAC gene family and further support the hypothesis that these genes are important for the plant responses to environmental stress.
组蛋白去乙酰化酶（HDACs）作为关键的表观遗传因子，在植物生长、发育的多个方面以及植物对非生物或生物胁迫的反应中抑制基因的表达。迄今为止，HDACs 的分子功能在拟南芥中得到了很好的描述，但尚未有关于大豆（Glycine max）中该基因家族的系统分析。
本研究从大豆基因组中鉴定出 28 个 HDAC 基因，它们不均匀地分布在 12 条染色体上。系统发育分析表明，GmHDACs 分为先前命名为 RPD3/HDA1、SIR2 和 HD2 的三个主要组。亚细胞定位分析表明，YFP 标记的 GmSRT4、GmHDT2 和 GmHDT4 主要定位于细胞核，而 GmHDA6、GmHDA13、GmHDA14 和 GmHDA16 则存在于细胞质和细胞核中。实时定量 PCR 显示，GmHDA6、GmHDA13、GmHDA14、GmHDA16 和 GmHDT4 在植物组织中广泛表达，而 GmHDA8、GmSRT2、GmSRT4 和 GmHDT2 在不同组织中表现出差异表达。有趣的是，我们测量了对几种非生物线索的响应中 GmHDACs 转录物积累的差异变化，表明这些表观遗传修饰因子可能是大豆对胁迫的动态转录响应的一部分。最后，我们表明先前报道与植物 HDACs 相关的组蛋白标记的水平可被冷和热调节。
我们在大豆中鉴定和分类了 28 个 HDAC 基因。我们的数据为 HDAC 基因家族的进化提供了见解，并进一步支持了这些基因对植物响应环境胁迫很重要的假说。
