Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou, 510642, China.
NHC Key Laboratory of Food Safety Risk Assessment, Chinese Academy of Medical Science Research Unit (No. 2019RU014), China National Center for Food Safety Risk Assessment, Beijing, 100022, PR China.
Anal Chim Acta. 2022 Aug 15;1221:340138. doi: 10.1016/j.aca.2022.340138. Epub 2022 Jun 30.
The coexistent pollution of multiple mycotoxins displays a synergistic toxicity effect that significantly threatens human health. Therefore, it is essential to establish a rapid detection method for multi-mycotoxins in food. In this study, red, green, and blue latex microspheres (LMs) were applied as the aflatoxin B (AFB), T-2 toxins (T-2), and zearalenone (ZEN) antibodies labeled tracer, respectively. Based on the principle of spatial resolution, a rainbow "traffic light" pattern latex microspheres lateral flow immunoassay (LMs-LFIA) integrated with a portable and user-friendly smartphone-based device was first developed to detect three kinds of mycotoxins simultaneously. The cut-off values of the method for AFB/T-2/ZEN in cereals were 1/15/40 μg kg, the limits of detection were 0.04/0.40/1.21 μg kg, respectively. The recoveries ranged from 82.1% to 107.5%, with the coefficient of variation from 3.0% to 8.1%. A parallel analysis in 26 naturally contaminated cereal samples was confirmed by commercial ELISA kits; the results showed a good correlation (R＞0.99), indicating the practical reliability of the rainbow LMs-LFIA. This method provided a visually enjoyable, portable, and sensitive detection mode for multi-target detection of mycotoxins or other small molecule hazard factors in food.
多种真菌毒素共存的污染呈现出协同毒性效应，这对人类健康构成了重大威胁。因此，建立食品中多类真菌毒素的快速检测方法至关重要。本研究分别将红色、绿色和蓝色乳胶微球（LMs）用作黄曲霉毒素 B（AFB）、T-2 毒素（T-2）和玉米赤霉烯酮（ZEN）抗体标记示踪剂。基于空间分辨率的原理，首次开发了一种集成了便携式和用户友好型智能手机的彩虹“交通灯”图案乳胶微球侧向流动免疫分析（LMs-LFIA），用于同时检测三种真菌毒素。该方法用于谷物中 AFB/T-2/ZEN 的截断值分别为 1/15/40μgkg，检测限分别为 0.04/0.40/1.21μgkg。回收率范围为 82.1%至 107.5%，变异系数为 3.0%至 8.1%。通过商业 ELISA 试剂盒对 26 个自然污染谷物样品进行平行分析，结果显示具有良好的相关性（R＞0.99），表明彩虹 LMs-LFIA 的实际可靠性。该方法为食品中多目标真菌毒素或其他小分子危害因子的检测提供了一种直观、便携和灵敏的检测模式。
