College of Forestry, Jiangxi Agricultural University, Nanchang, Jiangxi, China.
Institute of Biological Resources, jiangxi Academy of Sciences, Nanchang, Jiangxi, China.
PeerJ. 2022 Aug 2;10:e13836. doi: 10.7717/peerj.13836. eCollection 2022.
is rich in color, making it a well-known horticultural ornamental plant. However, little is known about the genes related to anthocyanin and carotenoid biosynthesis in . In this study, transcriptome sequencing was performed on eight different colors of . A total of 1.13 billion clean reads were obtained and assembled into 89,124 unigenes, which were then aligned with six databases, resulting in the identification of 54,823 annotated unigenes. Among these unigenes, 76 and 101 were involved in the biosynthetic pathway of carotenoids and anthocyanins, respectively. In addition, 505 transcription factors were revealed, which belonged to the , , , , and families. A total of 6,700 differentially expressed genes (DEGs) were obtained by comparative transcriptome analysis. Among them, 17 candidate unigenes (four carotenoid genes, seven anthocyanin genes, and six TFs) were specifically up-regulated for one or more colors of . Eight representative candidate unigenes were analyzed by qRT-PCR. The findings enrich the transcriptome database of lupine, and provide a rich molecular resource for research on the coloration mechanism of .
是一种富含花色苷和类胡萝卜素的植物，颜色丰富，是一种著名的园艺观赏植物。然而，关于 中花色苷和类胡萝卜素生物合成相关基因的研究却很少。本研究对 8 种不同颜色的 进行了转录组测序。共获得 11.3 亿条清洁reads，组装成 89124 条unigenes，然后与 6 个数据库进行比对，鉴定出 54823 个注释 unigenes。其中，76 个和 101 个分别参与了类胡萝卜素和花色苷的生物合成途径。此外，还揭示了 505 个转录因子，属于 、 、 、 和 家族。通过比较转录组分析共获得 6700 个差异表达基因（DEGs）。其中，17 个候选 unigenes（4 个类胡萝卜素基因、7 个花色苷基因和 6 个 TF）在一种或多种颜色中特异性上调。对 8 个代表性候选 unigenes进行了 qRT-PCR 分析。该研究丰富了羽扇豆的转录组数据库，为研究 花色形成机制提供了丰富的分子资源。
