Zhou Qiong, Zhu Yankun, Li Chun, Li Zhiqiang, Tang Zhe, Yuan Baohong, Wang Xiaodan, Zhang Shengqingyu, Wu Xinan
School of Public Health, Kunming Medical University, Kunming, People's Republic of China.
Department of Endocrinology, Yan'an Hospital Affiliated to Kunming Medical University, Kunming, People's Republic of China.
Diabetes Metab Syndr Obes. 2022 Jul 30;15:2277-2285. doi: 10.2147/DMSO.S373203. eCollection 2022.
Cathepsin L (CTSL) and B (CTSB) were lysosomal proteases, and their expression and activity contribute to the progression of inflammation in obese rodents. Our aim was to investigate CTSB and CTSL expression in omental adipose tissue (AT) of patients with obesity and to correlate CTSB and CTSL expression with proinflammatory cytokines (CCL-2, IL-6 and IL-1β).
A total of 12 patients without obesity (NOB) and 51 patients with obesity (OB) were involved in this study. Omental AT was collected from all the participants for RNA extraction. Expressions of CTSB, CTSL and proinflammatory cytokines (CCL-2, IL-6 and IL-1β) were qualified with qRT-PCR. BMI (body mass index) and metabolic parameters were measured.
The mRNA expression levels of both CTSB and CTSL were upregulated in the OB group (t = 2.693, P < 0.05; t = 2.849, <0.01) and were related to TC levels (Std.β=0.443, <0.05; Std.β=0.439, <0.05). However, only the CTSB level was related to BMI (Std.β=0.261, <0.05). In multiple regression analysis, CTSL was independently associated with CCL-2, IL-6 and IL-1β levels (Std.β=0.352-0.462, <0.05).
CTSB and CTSL gene expressions were elevated in the omental AT of OB group. CTSL, but not CTSB, was positively correlated with proinflammatory cytokines independently, suggesting that the dysregulation of CTSL may play a significant role in the inflammatory process.
组织蛋白酶L（CTSL）和B（CTSB）是溶酶体蛋白酶，它们的表达和活性促进肥胖啮齿动物炎症的进展。我们的目的是研究肥胖患者网膜脂肪组织（AT）中CTSB和CTSL的表达，并将CTSB和CTSL的表达与促炎细胞因子（CCL-2、IL-6和IL-1β）进行关联。
本研究共纳入12例非肥胖患者（NOB）和51例肥胖患者（OB）。收集所有参与者的网膜脂肪组织用于RNA提取。采用qRT-PCR检测CTSB、CTSL和促炎细胞因子（CCL-2、IL-6和IL-1β）的表达。测量体重指数（BMI）和代谢参数。
OB组中CTSB和CTSL的mRNA表达水平均上调（t = 2.693，P < 0.05；t = 2.849，<0.01），且与总胆固醇（TC）水平相关（标准β=0.443，<0.05；标准β=0.439，<0.05）。然而，只有CTSB水平与BMI相关（标准β=0.261，<0.05）。在多元回归分析中，CTSL与CCL-2、IL-6和IL-1β水平独立相关（标准β=0.352 - 0.462，<0.05）。
OB组网膜脂肪组织中CTSB和CTSL基因表达升高。CTSL而非CTSB与促炎细胞因子呈正相关，提示CTSL的失调可能在炎症过程中起重要作用。
