Wang Haili, Chen Xinhua, Chen Chunhai, Pan Ting, Li Mengyuan, Yao Lin, Li Xuefeng, Lu Qi, Wang Hongfeng, Wang Zhaohui
School of Acupuncture-Moxibustion and Tuina, Changchun University of Chinese Medicine, Changchun, Jilin, 130117, People's Republic of China.
Department of Acupuncture and Moxibustion, Affiliated Hospital of Changchun University of Chinese Medicine, Changchun, Jilin, 130021, People's Republic of China.
Diabetes Metab Syndr Obes. 2022 Jul 30;15:2265-2276. doi: 10.2147/DMSO.S374843. eCollection 2022.
The study objective was to investigate the effects of electroacupuncture performed at the he-sea and front-mu acupoints on the intestinal microflora and intestinal barrier in db/db mice and to explore the related mechanism in type 2 diabetes mellitus.
Db/m mice in the normal control group (NOC), electroacupuncture group (EA), metformin group (MET) and T2DM group (T2DM) were used as model controls, and db/db mice were used in all three groups, with 8 mice in each group. The treatment period was 2 weeks. Fasting blood glucose (FBG) and triglyceride (TG) levels were measured. Lipopolysaccharide (LPS) and tumor necrosis factor-α (TNF-α) levels were detected by enzyme-linked immune sorbent assay (ELISA). The ileal tissue was stained with hematoxylin-eosin staining (HE), and histopathological changes were observed under a light microscope. Illumina sequencing was used to analyze the V4 region of the 16S rRNA gene to evaluate the effect of EA on the intestinal flora.
Our results suggest that EA treatment can reduce the expression of diabetes-related markers, with an effect similar to that of metformin. After EA intervention, the abundance of Firmicutes and the ratio of Firmicutes to Bacteroidetes increased, while the abundances of Bacteroidetes and Eubacterium decreased. In addition, the serum levels of LPS and TNF-α in the electroacupuncture group were downregulated, and ileal tissue damage was alleviated under an electron microscope.
EA combined with acupoints can restore the intestinal flora structure, decrease the blood LPS level, reduce levels of inflammation, maintain the integrity of the intestinal barrier, and play a therapeutic role in the treatment of T2DM, mainly by increasing the abundance of Firmicutes and the ratio of Firmicutes to Bacteroidetes and decreasing the abundances of Bacteroidetes and Eubacterium.
本研究目的是探讨在合穴与募穴进行电针对db/db小鼠肠道微生物群和肠道屏障的影响，并探索2型糖尿病的相关机制。
将正常对照组（NOC）、电针组（EA）、二甲双胍组（MET）和2型糖尿病组（T2DM）的db/m小鼠作为模型对照，所有三组均使用db/db小鼠，每组8只。治疗期为2周。测量空腹血糖（FBG）和甘油三酯（TG）水平。采用酶联免疫吸附测定（ELISA）检测脂多糖（LPS）和肿瘤坏死因子-α（TNF-α）水平。用苏木精-伊红染色（HE）对回肠组织进行染色，在光学显微镜下观察组织病理学变化。采用Illumina测序分析16S rRNA基因的V4区域，以评估电针对肠道菌群的影响。
我们的结果表明，电针治疗可降低糖尿病相关标志物的表达，其效果与二甲双胍相似。电针干预后，厚壁菌门丰度及厚壁菌门与拟杆菌门的比例增加，而拟杆菌门和真杆菌属的丰度降低。此外，电针组血清LPS和TNF-α水平下调，电镜下回肠组织损伤减轻。
电针结合穴位可恢复肠道菌群结构，降低血LPS水平，减轻炎症水平，维持肠道屏障完整性，对2型糖尿病治疗发挥作用，主要是通过增加厚壁菌门丰度及厚壁菌门与拟杆菌门的比例，降低拟杆菌门和真杆菌属的丰度。
