Department of Acupuncture and Moxibustion, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200086, China.
Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Oxid Med Cell Longev. 2022 Jul 26;2022:3695848. doi: 10.1155/2022/3695848. eCollection 2022.
Human amniotic fluid stem cell-derived exosome (HuAFSC-exosome) transplantation is considered a promising treatment for premature ovarian failure (POF). However, its mechanism remains unclear. In this study, exosomes were isolated and enriched from HuAFSC subsets of CD44+/CD105+, and the exosomes were transplanted into a POF model and . Our results confirmed that the exosomes produced by CD44+/CD105+ HuAFSCs could achieve therapeutic effects in a mouse POF model. Our research also showed that CD44+/CD105+ HuAFSC-exosomes carrying miR-369-3p could specifically downregulate the expression of YAF2, inhibit the stability of PDCD5/p53, and reduce the apoptosis of ovarian granulosa cells (OGCs), thereby exerting therapeutic effects on POF. Knowledge of these mechanisms demonstrates that miRNAs carried by CD44+/CD105+ HuAFSC-exosomes are critical to the therapy of POF. This will be useful for the clinical application of stem cells.
人羊膜间充质干细胞来源的外泌体（HuAFSC-exosome）移植被认为是治疗卵巢早衰（POF）的一种有前途的方法。然而，其机制尚不清楚。在这项研究中，从 HuAFSC 亚群 CD44+/CD105+中分离和富集外泌体，并将外泌体移植到 POF 模型中。我们的结果证实，CD44+/CD105+ HuAFSC 产生的外泌体可以在小鼠 POF 模型中发挥治疗作用。我们的研究还表明，携带 miR-369-3p 的 CD44+/CD105+ HuAFSC-exosomes 可以特异性地下调 YAF2 的表达，抑制 PDCD5/p53 的稳定性，减少卵巢颗粒细胞（OGCs）的凋亡，从而对 POF 发挥治疗作用。对这些机制的认识表明，CD44+/CD105+ HuAFSC-exosomes 携带的 miRNAs 对 POF 的治疗至关重要。这将有助于干细胞的临床应用。
