Huerta-Olalde Ana Maria, Hernández-García Alejandra, López-Gómez Rodolfo, Fernández-Pavía Sylvia Patricia, Zavala-Páramo María Guadalupe, Salgado-Garciglia Rafael
Instituto de Investigaciones Químico-Biológicas, Edif. B3, Universidad Michoacana de San Nicolás de Hidalgo (UMSNH), Ciudad Universitaria, Ave. Francisco. J. Múgica S/N, C.P. 58030, Morelia, Michoacán, México.
Instituto de Investigaciones Agropecuarias y Forestales, UMSNH, Km. 9.5 Carretera Morelia-Zinapécuaro, C.P. 58880, Tarímbaro, Michoacán, México.
Plant Biotechnol (Tokyo). 2022 Jun 25;39(2):165-171. doi: 10.5511/plantbiotechnology.22.0312b.
Blackberry is an economically important crop in Mexico, and its yield is substantially reduced by gray mold, a disease caused by . One of the means to obtain -resistant plants is gamma irradiation. Shoot tips of in vitro-micropropagated blackberry plants ( 'Tupy') were irradiated with five doses of Cobalt-60 gamma radiation (0, 15, 30, 45, and 60 Gy) and cultured on Murashige and Skoog basal medium containing 1.0 mg l benzylaminopurine and 0.06 mg l indole-3-butyric acid (MSB medium). After 28 days of culture, survival was evaluated to determine mean lethal dose (LD), and 200 shoots were further irradiated at the determined LD (30.8 Gy). After 28 days, the surviving shoots were micropropagated on MSB medium for 60 days. Non-irradiated shoots were screened for the in vitro selection of resistant , exposing them to different concentrations of sterile culture filtrate of (0, 2, 4, 6, 8, and 10 g l) for 28 days to determine mean lethal concentration (LC), and the irradiated surviving shoots were further exposed to the determined LC (4.6 g l). Three surviving lines (rfgum5, rfgum6, and rfgum17) that did not present changes compared with the control shoots were micropropagated to obtain plantlets, which were further subjected to in vitro resistance assays using detached leaves inoculated with (1×10 spores ml). Plants of rfgum5 and rfgum6 mutant lines were highly resistant and presented similar growth to control plants. Therefore, this methodology is useful to obtain -resistant blackberry plants.
黑莓是墨西哥一种具有重要经济价值的作物，其产量因灰霉病而大幅降低，灰霉病是由[病原菌名称缺失]引起的一种病害。获得抗病植株的方法之一是伽马射线辐照。用五剂钴 - 60伽马射线（0、15、30、45和60 Gy）辐照离体微繁黑莓植株（‘图皮’）的茎尖，并在含有1.0 mg l苄氨基嘌呤和0.06 mg l吲哚 - 3 - 丁酸的Murashige和Skoog基本培养基（MSB培养基）上培养。培养28天后，评估存活率以确定平均致死剂量（LD），并以确定的LD（30.8 Gy）对200个茎尖进行进一步辐照。28天后，将存活的茎尖在MSB培养基上进行微繁60天。对未辐照的茎尖进行离体筛选以选择抗病[病原菌名称缺失]，将它们暴露于不同浓度的[病原菌名称缺失]无菌培养滤液（0、2、4、6、8和10 g l）中28天以确定平均致死浓度（LC），并将辐照后存活的茎尖进一步暴露于确定的LC（4.6 g l）。三个与对照茎尖相比未出现变化的存活株系（rfgum5、rfgum6和rfgum17）进行微繁以获得植株，这些植株进一步通过用[病原菌名称缺失]（1×10孢子 ml）接种离体叶片进行离体抗性测定。rfgum5和rfgum6突变株系的植株具有高度抗性，并且生长与对照植株相似。因此，这种方法对于获得抗病黑莓植株是有用的。
