1835年杜莫特（杜莫特分类法）（合叶苔科，叶苔目）叶绿体基因组的纳米孔测序
Nanopore sequencing of chloroplast genome of (L.) Dumort., 1835 (Scapaniaceae, Jungermanniales).
作者信息
Ciborowski Kamil, Skierkowski Bartosz, Żukowska Karolina, Krawczyk Katarzyna, Sawicki Jakub
机构信息
Department of Botany and Nature Protection, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
出版信息
Mitochondrial DNA B Resour. 2022 Aug 1;7(8):1424-1426. doi: 10.1080/23802359.2022.2107458. eCollection 2022.
We performed long-read sequencing of the (L.) Dumort. chloroplast genome with Oxford Nanopore Technology using improved flow cells (10.3). is a leafy liverwort living on rocks in or near water in mountains. The size of the complete plastome sequence is 117,651 bp long, the large single copy (LSC) region is 80,606 bp long, the small single copy (SSC) region is 19,401 bp long. The nanopore sequencing allowed for the distinction of inverted repeats A and B (IRA, IRB) which were not identical. Guanine cytosine (GC) ratio is 33.7% for the complete chloroplast genome, for LSC and SSC are 31.7% and 30.6%. The phylogeny reconstruction shows that is the sister to and .
我们使用改进的流动槽（10.3），通过牛津纳米孔技术对杜氏（L.）杜莫尔特叶绿体基因组进行了长读长测序。杜氏是一种生长在山区水边或附近岩石上的叶状地钱。完整的质体基因组序列长度为117,651 bp，大单拷贝（LSC）区域长度为80,606 bp，小单拷贝（SSC）区域长度为19,401 bp。纳米孔测序能够区分不相同的反向重复序列A和B（IRA，IRB）。完整叶绿体基因组的鸟嘌呤胞嘧啶（GC）比率为33.7%，LSC和SSC的GC比率分别为31.7%和30.6%。系统发育重建表明，杜氏是[此处原文缺失相关物种名]和[此处原文缺失相关物种名]的姐妹种。
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