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解析芦竹亚科的进化:质体基因组揭示密码子使用、诊断特征和超级条形码的甜蜜秘密。

Decoding Evolution of Rubioideae: Plastomes Reveal Sweet Secrets of Codon Usage, Diagnostides, and Superbarcoding.

机构信息

Department of Botany and Evolutionary Ecology, University of Warmia and Mazury in Olsztyn, Plac Łódzki 1, 10-727 Olsztyn, Poland.

出版信息

Genes (Basel). 2024 Apr 27;15(5):562. doi: 10.3390/genes15050562.

DOI:10.3390/genes15050562
PMID:38790191
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11121115/
Abstract

genus belongs to the Rubiaceae family, which consists of approximately 14,000 species. In comparison to its well-known relatives, the plastomes of the genus have not been explored so far. The plastomes of this genus have a typical, quadripartite structure, but differ in gene content, since the gene is missing in and . An evaluation of the effectiveness of using entire chloroplast genome sequences as superbarcodes for accurate plant species identification revealed the high potential of this method for molecular delimitation within the genus and tribe. The -UUC- region showed the biggest number of diagnostides (diagnostic nucleotides) which might be new potential barcodes, not only in , but also in other closely related genera. Relative synonymous codon usage (RSCU) appeared to be connected with the phylogeny of the Rubiaceae family, showing that during evolution, plants started preferring specific codons over others.

摘要

该属属于茜草科,约有 14000 种。与广为人知的近亲相比,该属的质体基因组尚未被探索。该属的质体基因组具有典型的四分体结构,但在基因含量上有所不同,因为 基因在 和 中缺失。评估使用整个叶绿体基因组序列作为超级条形码进行准确植物种鉴定的有效性,揭示了该方法在属和族内进行分子界定的巨大潜力。-UUC- 区域显示出最多的诊断核苷酸(诊断核苷酸),这些可能是新的潜在条形码,不仅在 中,而且在其他密切相关的属中也是如此。相对同义密码子使用(RSCU)似乎与茜草科的系统发育有关,表明在进化过程中,植物开始偏爱特定的密码子而不是其他密码子。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d4c/11121115/3a7c8db79e40/genes-15-00562-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d4c/11121115/28d82a84fc39/genes-15-00562-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d4c/11121115/cadc3e555c5b/genes-15-00562-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d4c/11121115/a090d6e9668d/genes-15-00562-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d4c/11121115/cbd01b0889ba/genes-15-00562-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d4c/11121115/80df7cd34404/genes-15-00562-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d4c/11121115/3f6508730007/genes-15-00562-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d4c/11121115/3a7c8db79e40/genes-15-00562-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d4c/11121115/28d82a84fc39/genes-15-00562-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d4c/11121115/cadc3e555c5b/genes-15-00562-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d4c/11121115/a090d6e9668d/genes-15-00562-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d4c/11121115/cbd01b0889ba/genes-15-00562-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d4c/11121115/80df7cd34404/genes-15-00562-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d4c/11121115/3f6508730007/genes-15-00562-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d4c/11121115/3a7c8db79e40/genes-15-00562-g007.jpg

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