VirScan：抗病毒抗体表位的高通量分析
VirScan: High-throughput Profiling of Antiviral Antibody Epitopes.
作者信息
Shrock Ellen L, Shrock Christine L, Elledge Stephen J
机构信息
Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.
Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Howard Hughes Medical Institute, Boston, MA 02115, USA.
出版信息
Bio Protoc. 2022 Jul 5;12(13). doi: 10.21769/BioProtoc.4464.
Profiling the specificities of antibodies can reveal a wealth of information about humoral immune responses and the antigens they target. Here, we present a protocol for VirScan, an application of the phage immunoprecipitation sequencing (PhIP-Seq) method for profiling the specificities of human antiviral antibodies. Accompanying this protocol is a video of the experimental procedure. VirScan and, more generally, PhIP-Seq are techniques that enable high-throughput antibody profiling by combining high-throughput DNA oligo synthesis and bacteriophage display with next-generation sequencing. In the VirScan method, human sera samples are screened against a library of peptides spanning the entire human viral proteome. Bound phage are immunoprecipitated and sequenced, identifying the viral peptides recognized by the antibodies. VirScan Is a powerful tool for uncovering individual viral exposure histories, mapping the epitope landscape of viruses of interest, and studying fundamental mechanisms of viral immunity. Graphical abstract.
分析抗体的特异性能够揭示大量关于体液免疫反应及其靶向抗原的信息。在此，我们介绍一种用于病毒扫描（VirScan）的方案，这是一种噬菌体免疫沉淀测序（PhIP-Seq）方法，用于分析人类抗病毒抗体的特异性。该方案配有实验过程的视频。病毒扫描以及更普遍的噬菌体免疫沉淀测序技术，通过将高通量DNA寡核苷酸合成、噬菌体展示与下一代测序相结合，实现了高通量抗体分析。在病毒扫描方法中，针对涵盖整个人类病毒蛋白质组的肽库对人类血清样本进行筛选。结合的噬菌体被免疫沉淀并测序，从而识别抗体所识别的病毒肽。病毒扫描是一种强大的工具，可用于揭示个体的病毒暴露史、绘制感兴趣病毒的表位图谱以及研究病毒免疫的基本机制。图形摘要。
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