Huang Ziru, Gunarathne Samarappuli Mudiyanselage Savini, Liu Wenwen, Zhou Yuwei, Jiang Yuqing, Li Shiqi, Huang Jian
School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China.
School of Healthcare Technology, Chengdu Neusoft University, Chengdu, China.
Front Bioinform. 2024 Sep 4;4:1424202. doi: 10.3389/fbinf.2024.1424202. eCollection 2024.
Phage-immunoprecipitation sequencing (PhIP-Seq) technology is an innovative, high-throughput antibody detection method. It enables comprehensive analysis of individual antibody profiles. This technology shows great potential, particularly in exploring disease mechanisms and immune responses. Currently, PhIP-Seq has been successfully applied in various fields, such as the exploration of biomarkers for autoimmune diseases, vaccine development, and allergen detection. A variety of bioinformatics tools have facilitated the development of this process. However, PhIP-Seq technology still faces many challenges and has room for improvement. Here, we review the methods, applications, and challenges of PhIP-Seq and discuss its future directions in immunological research and clinical applications. With continuous progress and optimization, PhIP-Seq is expected to play an even more important role in future biomedical research, providing new ideas and methods for disease prevention, diagnosis, and treatment.
噬菌体免疫沉淀测序（PhIP-Seq）技术是一种创新的高通量抗体检测方法。它能够对个体抗体谱进行全面分析。该技术显示出巨大的潜力，特别是在探索疾病机制和免疫反应方面。目前，PhIP-Seq已成功应用于各个领域，如自身免疫性疾病生物标志物的探索、疫苗开发和过敏原检测。多种生物信息学工具推动了这一进程的发展。然而，PhIP-Seq技术仍然面临许多挑战，还有改进的空间。在此，我们综述了PhIP-Seq的方法、应用和挑战，并讨论了其在免疫学研究和临床应用中的未来方向。随着不断的进步和优化，PhIP-Seq有望在未来的生物医学研究中发挥更重要的作用，为疾病的预防、诊断和治疗提供新的思路和方法。
