使用 VirScan 进行全面的噬菌体展示病毒抗体分析：在慢性免疫介导性疾病中的潜在应用。
Comprehensive phage display viral antibody profiling using VirScan: potential applications in chronic immune-mediated disease.
机构信息
Faculty of Medicine, Imperial College London, London, United Kingdom.
Ashford and St. Peter's Hospitals NHS Foundation Trust, Chertsey, United Kingdom.
出版信息
J Virol. 2024 Nov 19;98(11):e0110224. doi: 10.1128/jvi.01102-24. Epub 2024 Oct 21.
Abstract
Phage immunoprecipitation sequencing (PhIP-Seq) is a high-throughput platform that uses programmable phage display for serology. VirScan, a specific PhIP-Seq library encoding viral peptides from all known human viruses, enables comprehensive quantification of past viral exposures. We review its use in immune-mediated diseases (IMDs), highlighting its utility in identifying viral exposures in the context of IMD development. Finally, we evaluate its potential for precision medicine by integrating it with other large-scale omics data sets.
摘要
噬菌体免疫沉淀测序 (PhIP-Seq) 是一种高通量平台，它使用可编程噬菌体展示进行血清学研究。VirScan 是一种特定的 PhIP-Seq 文库，它编码来自所有已知人类病毒的病毒肽，可实现对过去病毒暴露的全面定量检测。我们回顾了它在免疫介导性疾病 (IMD) 中的应用，强调了它在确定 IMD 发展过程中病毒暴露方面的效用。最后，我们通过将其与其他大规模组学数据集整合来评估其在精准医学中的潜力。
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