Department of Biology, Emory University.
Department of Physics, Emory University.
J Vis Exp. 2022 Jul 22(185). doi: 10.3791/64027.
The nematode Caenorhabditis elegans is a model system for host-microbe and host-microbiome interactions. Many studies to date use batch digests rather than individual worm samples to quantify bacterial load in this organism. Here it is argued that the large inter-individual variability seen in bacterial colonization of the C. elegans intestine is informative, and that batch digest methods discard information that is important for accurate comparison across conditions. As describing the variation inherent to these samples requires large numbers of individuals, a convenient 96-well plate protocol for disruption and colony plating of individual worms is established.
秀丽隐杆线虫是一种用于研究宿主-微生物和宿主-微生物组相互作用的模式生物系统。迄今为止，许多研究使用批量消化而非单个蠕虫样本来定量该生物体内的细菌负荷。本文认为，在秀丽隐杆线虫肠道中细菌定植方面，个体间存在很大的可变性，这是有意义的，并且批量消化方法会丢弃在不同条件下进行准确比较很重要的信息。由于描述这些样本固有的变异需要大量个体，因此建立了一种方便的 96 孔板方案，用于单个蠕虫的裂解和菌落平板接种。
