Sin Jia En Valerie, Shen Ping, Huang Lifei, Wu Yuansheng, Chan Sheot Harn
National Centre for Food Science, Singapore Food Agency, 7 International Business Park, Singapore 609919, Singapore.
Department of Food Science & Technology, National University of Singapore, 2 Science Drive, Singapore 117543, Singapore.
Foods. 2024 Aug 18;13(16):2581. doi: 10.3390/foods13162581.
There has been a growing interest in the use of hemp as an animal feed ingredient considering its economic value and nutritional properties. However, there is a paucity of research regarding the safety of hemp-based animal feed currently. Thus, this raises safety concerns on the potential transfer of cannabinoids from hemp-based animal feed to animal products intended for human consumption and its health effects. As such, the detection and quantification of cannabinoids in meat and animal feeds would be desirable for monitoring purposes. In this study, a simple, rapid and sensitive method for the simultaneous quantification of four major cannabinoids (delta-9-tetrahydrocannabinol, cannabidiol, cannabinol and tetrahydrocannabinolic acid) in meat and animal feeds by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was successfully developed and validated. The method was selective and sensitive, achieving limits of detection and quantification for the four cannabinoids from 5 to 7 µg/kg and 15 to 21 µg/kg, respectively. The overall recovery with matrix-matched calibration curves for the cannabinoids ranged from 87-115%. The coefficients of variation were between 2.17-13.38% for intraday precision and 3.67-12.14% for inter-day precision. The method was subsequently applied to monitor cannabinoids in 120 meat and 24 animal feed samples. No cannabinoid was detected, suggesting no imminent food safety concerns arising from the potential incorporation of hemp and by-products in animal feed and nutrition under the promotion of sustainable agricultural practices.
考虑到大麻的经济价值和营养特性，人们对将其用作动物饲料成分的兴趣日益浓厚。然而，目前关于基于大麻的动物饲料安全性的研究较少。因此，这引发了人们对大麻素从基于大麻的动物饲料转移到供人类食用的动物产品及其健康影响的安全担忧。因此，为了进行监测，对肉类和动物饲料中的大麻素进行检测和定量是很有必要的。在本研究中，成功开发并验证了一种通过液相色谱-串联质谱法（LC-MS/MS）同时定量肉类和动物饲料中四种主要大麻素（δ-9-四氢大麻酚、大麻二酚、大麻酚和四氢大麻酚酸）的简单、快速且灵敏的方法。该方法具有选择性和灵敏性，四种大麻素的检测限和定量限分别为5至7μg/kg和15至21μg/kg。大麻素的基质匹配校准曲线的总体回收率在87%-115%之间。日内精密度的变异系数在2.17%-13.38%之间，日间精密度的变异系数在3.67%-12.14%之间。该方法随后被应用于监测120份肉类和24份动物饲料样品中的大麻素。未检测到大麻素，这表明在可持续农业实践的推动下，动物饲料和营养中潜在添加大麻及其副产品不会立即引发食品安全问题。
